Ribosomal Protein L24 Double Nickase Plasmid (h): sc-417652-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein L24 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ribosomal Protein L24 Double Nickase Plasmid (h) and Ribosomal Protein L24 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RPL24. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ribosomal Protein L24 Antibody (C-5): sc-518250
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein L24 Double Nickase Plasmid (h) | sc-417652-NIC | 20 µg | $410.00 |
RPL24 encodes ribosomal protein L24, an essential component of the 60S large ribosomal subunit that contributes to ribosome assembly and translational control. By supporting mRNA decoding capacity and nascent polypeptide synthesis, RPL24 influences core processes such as cell growth, proteostasis, and stress-adaptive translational programs. Perturbation of ribosomal proteins can elicit nucleolar stress and activate surveillance pathways including p53 signaling, linking ribosome biogenesis defects to altered cell-cycle regulation. Dysregulated ribosome production and translation are widely implicated in oncogenic transformation and ribosomopathy-like phenotypes, making RPL24 a relevant node for mechanistic studies of translational control in human cells.
Ribosomal Protein L24 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RPL24 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RPL24. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RPL24 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RPL24-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.