Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RANTES Double Nickase Plasmid (h): sc-401806-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RANTES Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RANTES Double Nickase Plasmid (h) and RANTES Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCL5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RANTES Antibody (F-11): sc-514019
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RANTES Double Nickase Plasmid (h) | sc-401806-NIC | 20 µg | $410.00 | |||
RANTES Double Nickase Plasmid (h2) | sc-401806-NIC-2 | 20 µg | $410.00 |
CCL5 encodes the chemokine RANTES, a secreted ligand that signals primarily through CCR1, CCR3, and CCR5 to direct chemotaxis of T cells, monocytes, eosinophils, and NK cells to sites of immune activation. RANTES-driven receptor signaling engages G protein–coupled pathways that modulate intracellular calcium flux, PI3K/AKT and MAPK cascades, integrin activation, and cytoskeletal remodeling, thereby shaping leukocyte recruitment and tissue inflammation. In human biology, CCL5 expression is tightly regulated by inflammatory transcriptional programs and contributes to coordinated immune cell trafficking within lymphoid and peripheral tissues. Dysregulated CCL5/RANTES signaling has been associated with chronic inflammatory conditions, autoimmune pathology, and tumor microenvironmental immune infiltration, making it a common target for mechanistic studies of immune regulation.
RANTES Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCL5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCL5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCL5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCL5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.