QIP1 Double Nickase Plasmid (h): sc-405442-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- QIP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- QIP1 Double Nickase Plasmid (h) and QIP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KPNA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: QIP1 Antibody (3D10): sc-101547
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QIP1 Double Nickase Plasmid (h) | sc-405442-NIC | 20 µg | $410.00 | |||
QIP1 Double Nickase Plasmid (h2) | sc-405442-NIC-2 | 20 µg | $410.00 |
KPNA4 encodes karyopherin subunit alpha 4 (QIP1), an importin-α family adaptor that recognizes classical nuclear localization signals and recruits importin-β to mediate protein translocation through the nuclear pore complex. By controlling nucleo-cytoplasmic trafficking, KPNA4 shapes transcriptional programs, cell-cycle progression, and stress-responsive signaling by regulating nuclear access of key transcription factors and regulatory proteins. Altered nuclear import dynamics involving KPNA4 have been linked to dysregulated proliferation, invasion, and genome maintenance pathways in cancer-relevant contexts, and can influence cellular responses to DNA damage and inflammatory cues. As a central node in nuclear transport, KPNA4 is frequently interrogated to dissect pathway wiring that depends on regulated nuclear localization.
QIP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KPNA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KPNA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KPNA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KPNA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.