Puromycin dihydrochloride CAS: 58-58-2
MF: C22H29N7O5•2HCl
MW: 544.43

Puromycin dihydrochloride (CAS 58-58-2)

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 120ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Puromycin dihydrochloride is rated 4.93 out of 5 by 14.
loading
5
13
4
1
3
0
2
0
1
0
別名: Puromycin dihydrochloride is also known as Stylomycin hydrochloride
アプリケーション: Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene
CAS 番号: 58-58-2
Purity: ≥98%
Molecular Weight: 544.43
Molecular Formula: C22H29N7O52HCl
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
* Refer to Certificate of Analysis for lot specific data (including water content).
loading

Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.
Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase.


参考文献

1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187

用法 :
For the selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles, proceed with puromycin selection as follows:

NOTE: The working puromycin concentration for selection in mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell line (see below). Use the lowest concentration that kills 100% of non-transfected cells in 3-5 days from the start of puromycin selection.


•48 hours post-transfection, aspirate the medium and replace with fresh medium containing puromycin at the appropriate concentration.

•Approximately every 2-3 days, aspirate and replace with freshly prepared selective media.

•Monitor the cells daily. Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days.

•Assay transfected cells.

NOTE: Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of puromycin should be titrated.
Titrating puromycin for selecting transfected cell lines:

•Plate 2 x 105 cells in each well of a 6-well plate containing 3 ml of the appropriate complete medium plus increasing concentrations of puromycin (i.e., 0, 1.0, 2.5, 5.0, 7.5, and 10.0 µg/ml)

•Replace with fresh selective medium after 2 days to remove dead cells.

•Examine the wells for viable cells every two days.

•Monitor the cells daily and observe the percentage of surviving cells. Optimum effectiveness should be reached in 1-4 days.

•The minimum antibiotic concentration to use is the lowest concentration that kills 100% of the cells in 3-5 days from the start of puromycin selection.
Appearance :
Powder
Physical State :
Solid
Derived From :
Streptomyces alboniger
Solubility :
Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 7.2 (~10 mg/ml).
保存方法 :
Desiccate at -20° C
Melting Point :
178-180° C
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
WGK Germany :
3
RTECS :
AU7355000
PubChem CID :
Merck Index :
14: 7943
MDL Number :
MFCD00012691
EC Number :
200-387-8
Beilstein Registry :
3853613
SMILES :
CN(C)C1=NC=NC2=C1N=CN2[[email protected]]3[[email protected]@H]([[email protected]@H]([[email protected]](O3)CO)NC(=O)[[email protected]@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Download SDS (MSDS)

分析表

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Puromycin dihydrochloride (CAS 58-58-2)  参考文献

他の人がPuromycin dihydrochloride (CAS 58-58-2)をどのように使用したかをご覧ください。 エントリをクリックして、PubMedエントリを表示します。 .

引用 1 to 10 of 269 合計

PMID: # 33436530  Li, F.|Zhang, J.|Chen, A.|Liao, R.|Duan, Y.|Xu, Y.|Tao, L.| et al. 2021. Aging (Albany NY). 13: 262-278.

PMID: # 33756106  Villa, E.|Sahu, U.|O'Hara, BP.|Ali, ES.|Helmin, KA.|Asara, JM.|Gao, P.|Singer, BD.|Ben-Sahra, I.| et al. 2021. Mol Cell.

PMID: # 33802790  Naffa, R.|Padányi, R.|Ignácz, A.|Hegyi, Z.|Jezsó, B.|Tóth, S.|Varga, K.|Homolya, L.|Hegedűs, L.|Schlett, K.|Enyedi, A.| et al. 2021. Cancers (Basel). 13:

PMID: # 33846462  Hamar, J.|Kültz, D.| et al. 2021. Sci Rep. 11: 7854.

PMID: # 34007175  Zhang, L.|Wang, M.|Zhu, Z.|Ding, C.|Chen, S.|Wu, H.|Yang, Y.|Che, F.|Li, Q.|Li, H.| et al. 2021. Int J Nanomedicine. 16: 3217-3240.

PMID: # 33440192  Sauter, MM.|Brandt, CR.| et al. 2021. Exp Eye Res. 108436.

PMID: # 33692342  Zhang, G.|Li, X.|Wu, L.|Qin, YX.| et al. 2021. Bone Res. 9: 16.

PMID: # 33671709  Pietrzak, J. et al. 2021. Pharmaceuticals (Basel, Switzerland). 14:

PMID: # 34088927  Lee, JC.|Yotis, DM.|Lee, JY.|Sarabusky, MA.|Shrum, B.|Champagne, A.|Ismail, OZ.|Tutunea-Fatan, E.|Leong, HS.|Gunaratnam, L.| et al. 2021. Sci Rep. 11: 11840.

PMID: # 33547545  Li, H.|Weng, Y.|Lai, L.|Lei, H.|Xu, S.|Zhang, Y.|Li, L.| et al. 2021. Mol Cell Biochem. 476: 2125-2134.

引用 1 to 10 of 269 合計
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 121ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;