Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
METTL7B Double Nickase Plasmid (h): sc-408340-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- METTL7B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- METTL7B Double Nickase Plasmid (h) and METTL7B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting METTL7B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: METTL7B Antibody (D-2): sc-398626
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
METTL7B Double Nickase Plasmid (h) | sc-408340-NIC | 20 µg | $410.00 |
METTL7B (methyltransferase-like 7B) encodes a putative S-adenosyl-L-methionine–dependent methyltransferase localized primarily to endomembrane compartments, with reported roles in regulating lipid-associated metabolism and cellular stress responses. METTL7B expression has been linked to pathways affecting membrane homeostasis, inflammatory signaling, and redox balance, consistent with its association with metabolic remodeling in diverse cell types. Altered METTL7B levels have been observed in multiple disease-relevant contexts, including cancer biology and neurodegeneration-associated transcriptional programs, making it a useful node for studying context-dependent gene regulation. Functional interrogation of METTL7B supports mechanistic studies of methylation-dependent protein or small-molecule modification and downstream effects on cell state.
METTL7B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the METTL7B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within METTL7B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt METTL7B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of METTL7B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.