MEK-2抗体(8E8) mouse, rat, human と canine 由来のMEK-2 WB と IPでの検出にはお勧めします

MEK-2抗体(8E8): sc-81473

MEK-2抗体(8E8)は、 2件のレビューによって、 5つ星のうち 4.0と 評価されています。
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データシート

    • MEK-2抗体 8E8はマウスモノクローナルIgG1です。50 µg/0.5 mlで提供
    • raised against N-terminus of MEK-2 of human origin
    • mouse, rat, human と canine 由来のMEK-2 WB と IPでの検出にはお勧めします
    • MEK-2 (A-1): sc-13159 MEK-2 抗体は、AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 と 790標識された抗体も提供できます。
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MEK-2 Antibody (8E8). This work is in progress.
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I am using MEK-2 (8E8): sc-81473 antibody for immunoprecipitation, how much antibody should I use?

質問者: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
回答者: Technical Support
投稿日: 2017-03-24
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によって、 5つ星のうち 3 と評価されています。 Positive western results when 8E8 tested with non Positive western results when 8E8 tested with non-transfected 293T, human MEK-2 transfected 293T and Jurkat whole cell lysates. -SCBT QC
投稿日: 2015-03-31
によって、 5つ星のうち 5 と評価されています。 Strong band clearly observed in western blot Strong band clearly observed in western blot analysis of MEK-2 expression in NIH/3T3 whole cell lysate. -SCBT QC
投稿日: 2013-10-14
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