m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for connexin 31.1 Antibody (H-9) for WB applications. This reagent is now offered in a bundle with connexin 31.1 Antibody (H-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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connexin 31.1 Antibody (H-9) is a high quality monoclonal connexin 31.1 antibody (also designated GJB5 antibody, gap junction protein beta 5 antibody, or CX31.1 antibody) suitable for the detection of the connexin 31.1 protein of mouse, rat and human origin. connexin 31.1 Antibody (H-9) is available as the non-conjugated anti-connexin 31.1 antibody. The connexin family of proteins form hexameric complexes, called connexons, that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino acid substitutions in the most highly conserved domains; the transmembrane and extracellular regions. Connexin 31.1, Cx31.1 or Gap junction beta-5 protein, is a 271 amino acid protein that is predominantly expressed in skin with lower expression in testis. Expression of connexin 31.1 is required for normal placental development in mice. Down-regulation of the connexin 31.1 gene correlates with head and neck squamous cell carcinomas (HNSCC) and therefore it may be a potential therapeutic target.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended starting dilution for Western blot for connexin 31.1 (H-9): sc-515690 monoclonal antibody?
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link:
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