CD40 Double Nickase Plasmid (m): sc-423444-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD40 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD40 Double Nickase Plasmid (m) and CD40 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cd40. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD40 Antibody (HM40-3): sc-20010
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD40 Double Nickase Plasmid (m) | sc-423444-NIC | 20 µg | $410.00 |
Mouse Cd40 encodes CD40, a TNF receptor superfamily member expressed on antigen-presenting cells and other immune subsets, where it mediates costimulatory communication with CD40L to coordinate adaptive immune responses. CD40 signaling engages TRAF adaptor proteins to activate canonical and noncanonical NF-κB, MAPK, and PI3K pathways, driving cytokine production, antigen presentation programs, and cell survival decisions. In B cells, CD40 contributes to germinal center formation, class-switch recombination, and affinity maturation, while in myeloid cells it modulates inflammatory activation and cross-talk with T cells. Dysregulated CD40 axis activity is implicated in autoimmune and chronic inflammatory phenotypes, transplant-related immune responses, and tumor immune microenvironment remodeling, making it a common target for mechanistic immunology studies.
CD40 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cd40 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cd40. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cd40 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cd40-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.