caspase-8抗体(2.1.24): sc-81657

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データシート

- caspase-8抗体 (2.1.24)はマウスモノクローナルIgG₁ κです。200 µg/mlで提供
- raised against full-length recombinant caspase-8 of human origin
- mouse, rat と human 由来のcaspase-8 WB と IPでの検出にはお勧めします
- caspase-8 (8CSP03): sc-56070 caspase-8 抗体は、AC, HRP, FITC, PE, Alexa Fluor^® 488, 594, 647, 680 と 790標識された抗体も提供できます。
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP は推奨セカンダリー検出試薬 caspase-8 抗体 (2.1.24) for WB applications. これらの試薬と caspase-8 抗体 (2.1.24) (下記の注文情報をご覧ください)とセットになっています。

関連項目

ほかの Caspase抗体、 caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5と caspase-6を含め
Caspase細胞応答の機能解析のためのChemCruz™ Chemical Caspase 阻害剤、 Caspase アクチベーター又は Caspase 基質

Anti-caspase-8 Antibody (2.1.24) - Western Blotting - Image 375939

Anti-caspase-8 Antibody (2.1.24) - Western Blotting - Image 353184

Anti-caspase-8 Antibody (2.1.24) - Western Blotting - Image 143488

Anti-caspase-8 Antibody (2.1.24) - Western Blotting - Image 46840

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Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

動物種	遺伝子名	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	CASP8	841	2q33.1	XM_005246885, XM_005246886, XM_005246887, XM_005246888, XM_005246889, XM_005246890, XM_005246891, XM_005246892, XM_005246893, XM_005246894, XM_005246895, XM_006712789, XM_006712790, XM_006712793, XM_011511969, NM_001080124, NM_001080125, NM_001228, NM_033355, NM_033356, NM_033358, NM_033357	Q14790	607271
Mouse	Casp8	12370	1 C1.3	XM_006495634, NM_001080126, NM_001277926, NM_009812	O89110	N/A

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オンラインプロトコル

What is the recommended secondary reagent to be used with sc-81657: caspase-8 (2.1.24) mouse monoclonal primary?

質問者: jenniferc

We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

回答者: Technical Support

投稿日: 2017-04-12

BOによって、 5つ星のうち 4 と評価されています。 Working well in Western Blot Working well in Western Blot, nice bands in in untreated and treated Jurkat whole cell lysates. -SCBT QC

投稿日: 2015-03-25

LRによって、 5つ星のうち 4 と評価されています。 Good specificity and signal strength with Good specificity and signal strength with Jurkat whole cell lysate. -SCBT QC

投稿日: 2015-02-27

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caspase-8抗体(2.1.24): sc-81657

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関連項目

What is the recommended secondary reagent to be used with sc-81657: caspase-8 (2.1.24) mouse monoclonal primary?