beta Tubulin抗体(D-10) mouse, rat と human 由来のβ Tubulin WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします

β Tubulin抗体(D-10): sc-5274

β Tubulin抗体(D-10)は、 83件のレビューによって、 5つ星のうち 4.7と 評価されています。
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データシート

    • 反-beta Tubulin抗体 D-10はマウスモノクローナルIgG2bβ Tubulin 抗体 です。200 µg/mlで提供
    • raised against a recombinant protein corresponding to amino acids 210-444 of β Tubulin of human origin
    • 反-beta Tubulin抗体 (D-10) mouse, rat と human 由来のβ Tubulin WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします
    • 反-beta Tubulin抗体 (D-10) IP用のアガロース、に共役での利用可能です。WB、IHC(P)とELISA用のHRP、IF、IHC(P)とFCM用のphycoerythrin または FITC、に共役での利用可能です。
    • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
    • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
    • WB,IHC(P)とELISA用のビオチンに共役での利用可能です。
    • β Tubulin (D-10): sc-5274無料の10 µgサンプルを受け取るには、ご連絡ください。

What is the lower band (~33kDa) in the western blot image shown on this webpage for sample (D)-KNRK whole cell lysate? I observed this band in my HeLa whole cell lysate as well.

質問者: Kayla08
Thank you for your question. Unfortunately this is not a band that we consistently see in our in house testing, so we have not looked into this non-specific band. We do not see it in Hela cells, so I am not sure what you could be seeing. The antibody does not cross-react with anything that should run at that size. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
回答者: Tech Service
投稿日: 2020-01-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

質問者: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
回答者: Tech Service 3
投稿日: 2016-12-21

Which isoform of beta tubulin does this antibody recognize?

質問者: eleni
Thank you for your question. Unfortunately the exact epitope of this antibody has not been mapped. It is raised against a recombinant protein corresponding to amino acids 210-444 of β Tubulin of human origin, accession#P07437. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
回答者: Tech Service
投稿日: 2017-05-09
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によって、 5つ星のうち 5 と評価されています。 It works very nicely We have used this antibody in the lab for 4 years, and it has always worked nicely. We use a 1:3000 dilution.
投稿日: 2021-04-02
によって、 5つ星のうち 5 と評価されています。 beta-tubulin Ab for WB Excellent antibody for WB dilution 1:50000 good for housekeeping protein of colon cancer cell lines
投稿日: 2021-03-23
によって、 5つ星のうち 2 と評価されています。 discreto funzionamento Questo anticorpo funziona discretamente su cellule fissate con metanolo freddo (-20°C); non funziona in cellule fissate in formalina
投稿日: 2021-03-18
によって、 5つ星のうち 5 と評価されています。 Great b-Tubulin antibody for Western This is a very good mouse monoclonal Ab for beta-Tubulin. We used it with 1:500 dilution and results were very satisfactory and clean. I recommend this antibody.
投稿日: 2019-07-30
によって、 5つ星のうち 2 と評価されています。 Works for HEK293T cells but not for Mouse ES cells I used 1:1000 dilution for cells lysates derived from HEK293T cells. Worked beautifully as an internal loading control. The data sheet says the antibody can recognise mouse, human and rat tubulin. Tried multiple dilutions in mouse ES cells never got a band.
投稿日: 2019-07-15
によって、 5つ星のうち 5 と評価されています。 good control antibody for Western blot We used the antibody for Western blot on whole cell lysate from HEK293T cells. It gives a clean band at the correct size. I recommend the antibody.
投稿日: 2019-05-17
によって、 5つ星のうち 5 と評価されています。 Quality of this antibody This antibody is very good, and works very well in our lab.
投稿日: 2019-01-15
によって、 5つ星のうち 2 と評価されています。 not as expected for a loading control antibody It works. However the quality is not as expected for b.actin that is often used as a loading control. Low quality if compared to other companies
投稿日: 2018-12-19
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