Benzonase® Nuclease 生理活性タンパク質: 核酸の完全消化用のエンドヌクレアーゼ

Benzonase® Nuclease (CAS 9025-65-4)

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別名: BenzNuclease
アプリケーション: Benzonase® Nuclease supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol is an endonuclease ideal for applications where complete digestion of nucleic acids is desirable
CAS 番号: 9025-65-4
Purity: ≥90%
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* Refer to Certificate of Analysis for lot specific data (including water content).
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Benzonase® Nuclease is a solubilized version of Benzonase® Nuclease Lyophilized Powder (sc-391121). The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.

Unit definition:
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.

Benzonase is a registered trademark of Merck KGaA.


参考文献

1. Miller, M.D., et al. 1994. Nat. Struct. Biol. 1: 461-468. PMID: 7664065

2. Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-3287. PMID: 8078761

3. Ball, T.K., et al. 1992. Nucleic Acids Res. 20: 4971-4974. PMID: 1329033

4. Ball, T.K., et al. 1987. Gene. 57: 183-192. PMID: 3319779

5. Janning, P., et al. 1994. Rapid Commun. Mass Spectrom. 8: 1035-1040. PMID: 7696699

6. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924

7. Herbert, B.R., et al. 1998. Electrophoresis. 19: 845-851. PMID: 9629925

用法 :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of Benzonase® Nuclease is 1.3×106 ~ 1.5×106 unit/mg protein.
Formulation :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
Physical State :
Liquid
Derived From :
Recombinant protein expressed in Escherichia coli
保存方法 :
Store at -20° C
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
WGK Germany :
1
MDL Number :
MFCD00131010
EC Number :
231-791-2

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Benzonase® Nuclease (CAS 9025-65-4)  参考文献

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引用 1 to 10 of 30 合計

PMID: # 34040255  Huang, S.|Zhu, S.|Kumar, P.|MacMicking, JD.| et al. 2021. Nature. 594: 424-429.

PMID: # 34582787  Wang, LL.|Serrano, C.|Zhong, X.|Ma, S.|Zou, Y.|Zhang, CL.| et al. 2021. Cell.

PMID: # 34375640  Jia, W.|Kim, SH.|Scalf, MA.|Tonzi, P.|Millikin, RJ.|Guns, WM.|Liu, L.|Mastrocola, AS.|Smith, LM.|Huang, TT.|Tibbetts, RS.| et al. 2021. J Biol Chem. 101049.

PMID: # 34709727  Liu, X.|Huuskonen, S.|Laitinen, T.|Redchuk, T.|Bogacheva, M.|Salokas, K.|Pöhner, I.|Öhman, T.|Tonduru, AK.|Hassinen, A.|Gawriyski, L.|Keskitalo, S.|Vartiainen, MK.|Pietiäinen, V.|Poso, A.|Varjosalo, M.| et al. 2021. Mol Syst Biol. 17: e10396.

PMID: # 33930289  Mendel, M.|Delaney, K.|Pandey, RR.|Chen, KM.|Wenda, JM.|Vågbø, CB.|Steiner, FA.|Homolka, D.|Pillai, RS.| et al. 2021. Cell.

PMID: # 32611815  Dong, C.|West, KL.|Tan, XY.|Li, J.|Ishibashi, T.|Yu, CH.|Sy, SMH.|Leung, JWC.|Huen, MSY.| et al. 2020. Proc Natl Acad Sci U S A. 117: 17019-17030.

PMID: # 31263572  Keskitalo, S.|Haapaniemi, EM.|Glumoff, V.|Liu, X.|Lehtinen, V.|Fogarty, C.|Rajala, H.|Chiang, SC.|Mustjoki, S.|Kovanen, P.|Lohi, J.|Bryceson, YT.|Seppänen, M.|Kere, J.|Heiskanen, K.|Varjosalo, M.| et al. 2019. NPJ Genom Med. 4: 14.

PMID: # 30692695  Charlesworth, CT.|Deshpande, PS.|Dever, DP.|Camarena, J.|Lemgart, VT.|Cromer, MK.|Vakulskas, CA.|Collingwood, MA.|Zhang, L.|Bode, NM.|Behlke, MA.|Dejene, B.|Cieniewicz, B.|Romano, R.|Lesch, BJ.|Gomez-Ospina, N.|Mantri, S.|Pavel-Dinu, M.|Weinberg, KI.|Porteus, MH.| et al. 2019. Nat. Med. 25: 249-254.

PMID: # 31135337  Bigot, N.|Day, M.|Baldock, RA.|Watts, FZ.|Oliver, AW.|Pearl, LH.| et al. 2019. Elife. 8:

PMID: # 31163614  Villafañez, F.|Gottifredi, V.|Soria, G.| et al. 2019. High Throughput. 8:

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