Apolipoprotein A I/APOA1抗体(B-10) human由来のapoA-I WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします

Apolipoprotein A I/APOA1抗体(B-10): sc-376818

Apolipoprotein A I/APOA1抗体(B-10)は、 10件のレビューによって、 5つ星のうち 4.5と 評価されています。
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データシート

    • 反-Apolipoprotein A I/APOA1抗体 B-10はマウスモノクローナルIgG2bApolipoprotein A I/APOA1 抗体 です。200 µg/mlで提供
    • human由来の全長apoA-Iを表すアミノ酸1-267に対応します
    • Apolipoprotein A I/APOA1抗体 (B-10) human由来のapoA-I WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします
    • 反-Apolipoprotein A I/APOA1抗体 (B-10) IP用のアガロース、に共役での利用可能です。WB、IHC(P)とELISA用のHRP、IF、IHC(P)とFCM用のphycoerythrin または FITC、に共役での利用可能です。
    • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
    • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
    • Apolipoprotein A I/APOA1 (B-10): sc-376818無料の10 µgサンプルを受け取るには、ご連絡ください。
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP は推奨セカンダリー検出試薬 Apolipoprotein A I/APOA1 抗体 (B-10) for WB and IHC(P) applications. これらの試薬と Apolipoprotein A I/APOA1 抗体 (B-10) (下記の注文情報をご覧ください)とセットになっています。
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Do you know of any cases, where the antibody was used to bind to the ApoA1-derived membrane scaffold protein?

質問者: Umbach27
Thank you for your question. We only have data for the detection of apoA-I of human origin in WB, IP, IF, IHC(P) and ELISA applications.
回答者: Tech Support Europe
投稿日: 2020-12-31

Is antigen retrieval required with working with paraffin-embedded sections?

質問者: AbPolly
Thank you for your question. Yes, we recommend performing antigen retrieval when using this antibody for IHC(P). Our recommended method is citrate buffer, pH 6, and heat. Our full protocol can be found on the website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
回答者: Technical Support 13
投稿日: 2017-02-09

Can we purchase following two items from vendors in India right now? are these two items are available in santa cruz right now? sc-376818 and sc-516102

質問者: saikatm91
Thank you for your question. This primary antibody and mouse binding protein are typically available in stock, however please inquire with our Customer Care Department for the most up to date information about product availability. Both products are currently available to be ordered through one of our authorized distributors in India. A link to search for contact information for our distributors is provided below. https://www.scbt.com/scbt/resources/distributors
回答者: Technical Support
投稿日: 2017-01-10

can sc-7297 is being uesed as secondery antibody for sc-376818

質問者: saikatm91
Thank you for your question. No, it can not be used as a secondary antibody. sc-7297 is the catalog number for a mouse monoclonal primary antibody against HCAM (DF1485). And sc-376818 is the catalog number for a different mouse monoclonal primary antibody against apoA-I (B-10). A list of recommended secondary antibodies to be used with this primary antibody can be found on the product page. Choose which application you are using the primary antibody in, and a list of products is provided.
回答者: Technical Support
投稿日: 2017-01-05
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によって、 5つ星のうち 4 と評価されています。 APOA1 This antibody is good,the bands were clear but had some unnecerssary bands
投稿日: 2017-08-25
によって、 5つ星のうち 5 と評価されています。 Clean and good sensitivity Produced a single band at expected MW in HepG2 cell extract and human kidney lysate. No protein detected in HEK293, possibly the protein is not expressed in this cell line?
投稿日: 2017-07-24
によって、 5つ星のうち 5 と評価されています。 Apolipoprotein A1 antibody for human samples I used this antibody for the human samples and used for immunoblotting as well as IHC both i found nice results.
投稿日: 2017-03-03
によって、 5つ星のうち 5 と評価されています。 functional ab ApoE in conditioned media of primary astrocytes from human apoE3/3 and apoE4/4 heterozygous mice was analyzed. Two bands were observed. These 2 bands were also observed when another anti-ApoE antibody (Non-sc) was used
投稿日: 2017-02-16
によって、 5つ星のうち 5 と評価されています。 Good for detecting the human protein A good choice for apoA detection. It provides clear and strong signal in blotting and staining procedures. This product has been published with.
投稿日: 2017-01-27
によって、 5つ星のうち 1 と評価されています。 Could not detect anything on my blot or IP Could not detect anything on my blot or IP.
投稿日: 2015-03-27
によって、 5つ星のうち 5 と評価されています。 Worked well in formalin fixed Worked well in formalin fixed, paraffin-embedded human kidney tissue for IHC. -SCBT QC
投稿日: 2015-03-19
によって、 5つ星のうち 5 と評価されています。 Immunofluorescence staining of formalin Immunofluorescence staining of formalin-fixed HepG2 cells shows golgi apparatus localization. -SCBT QC
投稿日: 2015-02-11
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