ZHX1 Lentiviral Activation Particles (h): sc-407666-LAC

Zinc fingers and homeoboxes protein 1 (ZHX1) is a nuclear transcription factor that contains homeobox and zinc-finger motifs and participates in gene expression programs controlling cell fate decisions, differentiation, and proliferation. ZHX1 commonly functions as a transcriptional repressor through interactions with other nuclear factors and modulation of chromatin-associated regulatory complexes, influencing pathways linked to development and lineage specification. Altered ZHX1 expression has been reported across multiple tumor contexts and in studies of immune and neuronal biology, supporting its relevance to cancer-associated transcriptional networks and tissue homeostasis. These features make ZHX1 a useful target for dissecting endogenous transcriptional control mechanisms and downstream pathway remodeling in human cell models.

ZHX1 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient ZHX1 upregulation across a broader range of human cell types.

ZHX1 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the ZHX1 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous ZHX1 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native ZHX1 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.