XRCC1 Double Nickase Plasmid (m): sc-423738-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XRCC1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XRCC1 Double Nickase Plasmid (m) and XRCC1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Xrcc1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XRCC1 Double Nickase Plasmid (m) | sc-423738-NIC | 20 µg | $410.00 |
Mouse Xrcc1 encodes XRCC1, a scaffold protein that coordinates base excision repair and single-strand break repair by assembling factors such as DNA ligase III, POLβ, and PARP1 at sites of damage. XRCC1 supports genome maintenance during replication and transcription by promoting timely processing and sealing of DNA strand interruptions, thereby limiting chromosomal aberrations and replication stress. Disruption of XRCC1-dependent repair elevates DNA damage signaling and can sensitize cells to endogenous oxidative lesions and genotoxic insults, linking the pathway to mechanisms of mutagenesis and genome instability relevant to disease-associated phenotypes in diverse tissues.
XRCC1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Xrcc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Xrcc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Xrcc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Xrcc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.