Anticorpo XBP-1 (F-4) raccomandato per il rilevamento di XBP-1 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo XBP-1 (F-4): sc-8015

Anticorpo XBP-1 (F-4) valutazione 3.8 di 5 di 27.
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Schede tecniche

    • Anti-XBP-1 Antibody F-4 è un monoclonale di topo IgG1 κ XBP-1 antibody, citato in 36 pubblicazioni, fornito in 200 µg/ml
    • generato contro aminoacidi 76-263 mapping al C-terminus dell´ XBP-1 di origine mouse
    • XBP-1 Antibody (F-4) é raccomandato per il rilevamento di XBP-1 di origine mouse, rat e human in WB, IP, IF e ELISA
    • XBP-1 Antibody (F-4) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-8015 X, 200 µg/0.1 ml)
    • Contattaci per ricevere un FREE 10 µg sample di XBP-1 (F-4): sc-8015.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for XBP-1 Antibody (F-4) for WB applications. These reagents are now offered in bundles with XBP-1 Antibody (F-4) (see ordering information below).
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Hi, I am looking for XBP1 antibody for Chip assay with mouse cells. XBP1 antibody (M186) was discontinued. Do you have any data demonstrating XBP-1 Antibody (F-4) sc-8015 works well for Chip assay with mouse cells? Any guarantee?Thank you.

Richiesta da: Toru
Thank you for your question. This antibody has not yet been tested in a ChIP application, and there are no references that we are aware of at this time for ChIP. It is, however, warrantied for ChIP use.
Risposta da: Tech Service
Data di pubblicazione: 2019-06-10

Will this recognize human XBP-1?

Richiesta da: jmb1
Thank you for your question. The sc-8015 is for detection of XBP-1 of human, mouse and rat origin.
Risposta da: Technical Support
Data di pubblicazione: 2019-02-10

When should the TransCruz reagent sc-8015 X be used instead of the standard form sc-8015?

Richiesta da: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Risposta da: Technical Support 9
Data di pubblicazione: 2017-01-17
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Rated 1 di 5 di da Non funziona per IHC I was interested in immunohistochemical revelation of this protein, but unfortunately (as stated in the datasheet), I can’t achive a signal on FFPE paraffined section of reactive tonsil (physical antigen retrieval CITRATE pH6.0 or EDTA pH8.0 using microwave/water bath or chimical antigen retrival using protease)
Data di pubblicazione: 2021-06-14
Rated 4 di 5 di da Works as portrayed The antibody showed a band at 55 Kda and also a small band at 43. Had to incubate the primary at room temperature overnight to get a band
Data di pubblicazione: 2018-10-09
Rated 3 di 5 di da It is not so good I didn't find the band which I was want to, and the background is not clear.
Data di pubblicazione: 2018-02-14
Rated 3 di 5 di da good This XBP-1 antibody works for western but has very strong nonspecific bands
Data di pubblicazione: 2018-02-02
Rated 2 di 5 di da Unspecific bands on mouse heart lysates Unfortunately I obtained many unspecific bands on mouse heart lysates, therefor it is difficult to determine the right bands.
Data di pubblicazione: 2017-11-23
Rated 1 di 5 di da Skeletal muscle immunostaining This antibody did not work for the immunostaining of frozen rat skeletal muscle sections.
Data di pubblicazione: 2017-11-22
Rated 3 di 5 di da the band is not very clear I used this antibody with a dilution of 1:200. However, the band was not very clear.
Data di pubblicazione: 2017-10-23
Rated 5 di 5 di da 功能不错 我一个月前申请了改抗体的试用装,很高兴能提供试用反馈,这个抗体还不错,洗脱过的膜上也杂处条带了,虽然不是很清晰,但是没有杂带,特异性不错。
Data di pubblicazione: 2017-09-27
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