Anti-XBP-1 Antibody F-4 è un monoclonale di topo IgG1 κ XBP-1 antibody, citato in 36 pubblicazioni, fornito in 200 µg/ml
generato contro aminoacidi 76-263 mapping al C-terminus dell´ XBP-1 di origine mouse
XBP-1 Antibody (F-4) é raccomandato per il rilevamento di XBP-1 di origine mouse, rat e human in WB, IP, IF e ELISA
XBP-1 Antibody (F-4) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-8015 X, 200 µg/0.1 ml)
Contattaci per ricevere un FREE 10 µg sample di XBP-1 (F-4): sc-8015.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for XBP-1 Antibody (F-4) for WB applications. These reagents are now offered in bundles with XBP-1 Antibody (F-4) (see ordering information below).
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XBP-1 Antibody (F-4) is a high quality monoclonal XBP-1 antibody (also designated XBP1 antibody) suitable for the detection of the XBP-1 protein of mouse, rat and human origin. XBP-1 Antibody (F-4) is available as both the non-conjugated anti-XBP-1 antibody form, as well as multiple conjugated forms of anti-XBP-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The X-box binding protein-1 (XBP-1 or hXBP-1), also designated tax-responsive element-binding protein 5 (TREB5) in mouse and human, or hepatocarcinogenesis-related transcription factor (HTF) in rat, belongs to the basic region/leucine zipper (bZIP) family of transcription factors. XBP-1 was first characterized as a protein that binds to the HLA-DRα promoter in B cells. XBP-1 recognizes the cAMP responsive element (CRE) in enhancers of human T cell leukemia virus and major histocompatibility complex class II genes and activates transcription of these genes. It is expressed at high levels in developing bone and its levels are modulated during osteoblast development, suggesting a role in regulation of expression of osteoblast-specific genes. In addition to binding to CRE sequences, XBP-1 has been shown to bind to TPA response elements (TREs).
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hi, I am looking for XBP1 antibody for Chip assay with mouse cells. XBP1 antibody (M186) was discontinued. Do you have any data demonstrating XBP-1 Antibody (F-4) sc-8015 works well for Chip assay with mouse cells? Any guarantee?Thank you.
Richiesta da: Toru
Thank you for your question. This antibody has not yet been tested in a ChIP application, and there are no references that we are aware of at this time for ChIP. It is, however, warrantied for ChIP use.
Risposta da: Tech Service
Data di pubblicazione: 2019-06-10
Will this recognize human XBP-1?
Richiesta da: jmb1
Thank you for your question. The sc-8015 is for detection of XBP-1 of human, mouse and rat origin.
Risposta da: Technical Support
Data di pubblicazione: 2019-02-10
When should the TransCruz reagent sc-8015 X be used instead of the standard form sc-8015?
Richiesta da: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Rated 1 di
Non funziona per IHCI was interested in immunohistochemical revelation of this protein, but unfortunately (as stated in the datasheet), I can’t achive a signal on FFPE paraffined section of reactive tonsil (physical antigen retrieval CITRATE pH6.0 or EDTA pH8.0 using microwave/water bath or chimical antigen retrival using protease)
Data di pubblicazione: 2021-06-14
Rated 4 di
Works as portrayedThe antibody showed a band at 55 Kda and also a small band at 43. Had to incubate the primary at room temperature overnight to get a band
Data di pubblicazione: 2018-10-09
Rated 3 di
It is not so goodI didn't find the band which I was want to, and the background is not clear.
Data di pubblicazione: 2018-02-14
Rated 3 di
goodThis XBP-1 antibody works for western but has very strong nonspecific bands
Data di pubblicazione: 2018-02-02
Rated 2 di
Unspecific bands on mouse heart lysatesUnfortunately I obtained many unspecific bands on mouse heart lysates, therefor it is difficult to determine the right bands.
Data di pubblicazione: 2017-11-23
Rated 1 di
TK IN da
Skeletal muscle immunostainingThis antibody did not work for the immunostaining of frozen rat skeletal muscle sections.
Data di pubblicazione: 2017-11-22
Rated 3 di
the band is not very clearI used this antibody with a dilution of 1:200. However, the band was not very clear.
Data di pubblicazione: 2017-10-23
Rated 5 di
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