WC4 Antibody (CC57): sc-101844
- WC4 Antibody (CC57) is a mouse monoclonal IgG1 κ WC4 antibody provided at 200 µg/ml
- raised against mesenteric lymph node cells of bovine origin
- WC4 Antibody (CC57) is recommended for detection of WC4 of bovine, ovine and caprine origin by WB, IP, IF and FCM
- Anti-WC4 Antibody (CC57) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for WC4 Antibody (CC57) for WB applications. These reagents are now offered in bundles with WC4 Antibody (CC57) (see ordering information below).
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WC4 Antibody (CC57) is a mouse monoclonal IgG1 kappa light chain antibody that detects WC4 protein of ovine, caprine, and bovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). WC4 (CC57) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WC4, also known as workshop cluster 4, is a transmembrane glycoprotein that plays a crucial role in the immune response by being selectively expressed on the surface of B-lymphocytes. WC4 is essential for the activation of intracellular signaling pathways, including the Ras and phosphatidylinositol 3-kinase pathways, which are vital for B-cell proliferation and differentiation. Activation of WC4 leads to the cross-linking of immunoglobulin chains on the B-cell membrane, facilitating the recruitment of Src family protein tyrosine kinases that further enhance B-cell signaling. WC4 forms functional complexes with other B-lymphocyte surface proteins such as integrin β1, CD21, and CD81, which are integral to regulating B-cell development and function. Expression of WC4 is maintained throughout B-cell maturation and persists even as B-cells differentiate into plasma cells, highlighting its importance in sustaining immune responses. Bovine WC4 has been identified as a homolog of CD19, reinforcing its significance in B-cell biology and its potential applications in immunological research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
WC4 Antibody (CC57) References:
- Structure of the genes encoding the CD19 antigen of human and mouse B lymphocytes. | Zhou, LJ., et al. 1992. Immunogenetics. 35: 102-11. PMID: 1370948
- CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. | Carter, RH. and Fearon, DT. 1992. Science. 256: 105-7. PMID: 1373518
- Basal expression studies of cystatins during specific growth stages of wheat spikes for defining their possible role in differential and stage dependent immunity against Karnal bunt (Tilletia indica). | Purwar, S., et al. 2010. Mol Biol Rep. 37: 1377-89. PMID: 19347606
- Individual antigens of cattle. Monoclonal antibodies reacting with bovine B cells (BoWC3, BoWC4 and BoWC5). | Naessens, J. and Howard, CJ. 1991. Vet Immunol Immunopathol. 27: 77-85. PMID: 2021068
- B-cell restricted saporin immunotoxins: activity against B-cell lines and chronic lymphocytic leukemia cells. | Bregni, M., et al. 1989. Blood. 73: 753-62. PMID: 2465042
- Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily. | Tedder, TF. and Isaacs, CM. 1989. J Immunol. 143: 712-7. PMID: 2472450
- Tissue-specific expression of the human CD19 gene in transgenic mice inhibits antigen-independent B-lymphocyte development. | Zhou, LJ., et al. 1994. Mol Cell Biol. 14: 3884-94. PMID: 7515149
- Analysis of bovine B cell reactive monoclonal antibodies. | Mukwedeya, DT., et al. 1996. Vet Immunol Immunopathol. 52: 285-94. PMID: 8896217
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WC4 Antibody (CC57) | sc-101844 | 200 µg/ml | $322.00 | |||
WC4 Antibody (CC57): m-IgG Fc BP-HRP Bundle | sc-527074 | 200 µg Ab; 10 µg BP | $361.00 | |||
WC4 Antibody (CC57): m-IgG1 BP-HRP Bundle | sc-532447 | 200 µg Ab; 20 µg BP | $361.00 | |||
WC4 Antibody (CC57) AC | sc-101844 AC | 500 µg/ml, 25% agarose | $424.00 | |||
WC4 Antibody (CC57) HRP | sc-101844 HRP | 200 µg/ml | $322.00 | |||
WC4 Antibody (CC57) FITC | sc-101844 FITC | 200 µg/ml | $336.00 | |||
WC4 Antibody (CC57) PE | sc-101844 PE | 200 µg/ml | $349.00 | |||
WC4 Antibody (CC57) Alexa Fluor® 488 | sc-101844 AF488 | 200 µg/ml | $364.00 | |||
WC4 Antibody (CC57) Alexa Fluor® 546 | sc-101844 AF546 | 200 µg/ml | $364.00 | |||
WC4 Antibody (CC57) Alexa Fluor® 594 | sc-101844 AF594 | 200 µg/ml | $364.00 | |||
WC4 Antibody (CC57) Alexa Fluor® 647 | sc-101844 AF647 | 200 µg/ml | $364.00 | |||
WC4 Antibody (CC57) Alexa Fluor® 680 | sc-101844 AF680 | 200 µg/ml | $364.00 | |||
WC4 Antibody (CC57) Alexa Fluor® 790 | sc-101844 AF790 | 200 µg/ml | $364.00 |