Anticorpo Vitamin D Receptor/VDR (D-6) raccomandato per il rilevamento di VDR di origine mouse, rat e human in WB, IP, IF e IHC(P)

Anticorpo Vitamin D Receptor/VDR (D-6): sc-13133

Anticorpo Vitamin D Receptor/VDR (D-6) valutazione 4.6 di 5 di 30.
  • y_2021, m_9, d_20, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_29
  • loc_it_IT, sid_13133, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 136ms
  • REVIEWS, PRODUCT
loading
5
20
4
9
3
1
2
0
1
0

Schede tecniche

    • Anti-Vitamin D Receptor/VDR Antibody D-6 è un monoclonale di topo IgG2a κ Vitamin D Receptor/VDR antibody, citato in 364 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 344-424 di VDR di origine human
    • Vitamin D Receptor/VDR Antibody (D-6) é raccomandato per il rilevamento di VDR di origine mouse, rat e human in WB, IP, IF e IHC(P)
    • Vitamin D Receptor/VDR Antibody (D-6) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • disponibile coniugato alla biotina per WB, IHC(P) e ELISA
    • reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-13133 X, 200 µg/0.1 ml)
    • Contattaci per ricevere un FREE 10 µg sample di Vitamin D Receptor/VDR (D-6): sc-13133.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Vitamin D Receptor/VDR Antibody (D-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Vitamin D Receptor/VDR Antibody (D-6) (see ordering information below).
loading

Hi there, since the VDR has different isoforms, I am wondering the amino acids sequence for VDR this antibody corresponding. Thanks!

Richiesta da: Fay Liu
Thank you for your question. VDR Antibody (D-6): sc-13133 was raised against amino acids 344-424 of VDR isoform 1 of human origin. This is the corresponding amino acid sequence: PGVQDAA LIEAIQDRLS NTLQTYIRCR HPPPGSHLLY AKMIQKLADL RSLNEEHSKQ YRCLSFQPEC SMKLTPLVLE VFGN Please do not hesitate to contact our technical service directly if you have additional questions.
Risposta da: Tech Support Europe
Data di pubblicazione: 2019-06-05

does D6 vitamin d receptor antibody cross react with monkey (macaques) for immunohistochemistry

Richiesta da: schristak
Thank you for your question. This antibody has not been tested on monkey samples in house, so it is not technically recommended or warrantied for that use, however, we do have publications in monkey (rhesus) samples.
Risposta da: Tech Service
Data di pubblicazione: 2019-04-09

Hello, I have been working with another VDR and I am not certain I am fully appreciating that VDR. Hence I am interested in the "Contact us to receive a FREE 10 µg sample of VDR (D-6): sc-13133." offer of getting 10 ug for free. Is it possible?

Richiesta da: hanna abrahamsson
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Risposta da: SCBT Heidelberg Support
Data di pubblicazione: 2018-12-14

Can Santa Cruz provide VDR IHC images (performed using D-6 sc-13133 ab) with staining intensity (score) of 1 to 4 and % positive stained cells (score) with 1-4 (preferably in FFPE colorectal tumor tissue samples) ?

Richiesta da: Sanbc
Thank you for the question. Please note that this product is sold for research or laboratory use only and it must not been used for medical diagnostics. All available IHC(P) images for this product will be published upon availability on our product website. We currently show staining of human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells, human skin tissue showing nuclear and cytoplasmic staining of keratinocytes, fibroblasts, Langerhans cells and melanocytes and human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Risposta da: Technical Support Europe
Data di pubblicazione: 2018-01-10

I got a double band when I used this antibody, any idea what the other band could be? Thanks

Richiesta da: Reham
Thank you very much for your inquiry. Please understand that we do not interpret customer data, since we do not have a detailed knowledge about your experiment and how the data was produced. Therefore our interpretation of the data could lead to a wrong direction without knowing all the relevant details. However, Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Common factors include: 1. Post-translational modification - e.g. phosphorylation, glycosylation etc, which increases the size of the protein. 2. Post-translation cleavage - e.g. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. pro-caspases. 3. Splice variants - alternative splicing may create different sized proteins produced from the same gene. 4. Relative charge - the composition of amino acids (charged vs non-charged). 5. Multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
Risposta da: Technical Support Europe
Data di pubblicazione: 2021-05-09

What is VDR D-6 best diluted in?

Richiesta da: Daraaaa
Thank you for your question. What it is best diluted in depends on the application you plan on using it for. For Western blot, we would recommend diluting it in 5% milk with TBS-T. For IF/IHC, we would recommend diluting it in blocking serum. You can find more information in the protocol you plan on following: https://www.scbt.com/scbt/resources/protocols
Risposta da: Tech Service
Data di pubblicazione: 2017-05-25

Hello, Does this ab work fine when it is conjugated to a regular anti-mouse IgG? Thanks!

Richiesta da: MMac
Thank you for your question. Yes, this antibody can be used with a conventional anti-mouse IgG secondary or our Mouse IgG Binding Protein, both are appropriate.
Risposta da: Technical Service
Data di pubblicazione: 2017-04-24

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Richiesta da: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-13133 is available directly conjugated to FITC, AF488, and AF647.
Risposta da: TechService7
Data di pubblicazione: 2021-01-21
  • y_2021, m_9, d_20, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_9
  • loc_it_IT, sid_13133, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 154ms
  • QUESTIONS, PRODUCT
Rated 5 di 5 di da Worked well in human and mouse muscle tissue. I used this antibody for IHC and got good results following published protocols. I used the unconjugated primary antibody and had to amplify for the human samples, but the mouse samples worked well going straight into secodary.
Data di pubblicazione: 2021-01-25
Rated 3 di 5 di da low dilution I needed to use a low dilution to make it work (1:50)
Data di pubblicazione: 2020-09-22
Rated 5 di 5 di da Buen anticuerpo Tiene una alta especificidad, en wblot da una muy buena señal y se puede reutilizar varias veces.
Data di pubblicazione: 2020-09-01
Rated 5 di 5 di da very good antibody This antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Data di pubblicazione: 2019-01-02
Rated 5 di 5 di da Best Antibody We have tried this antibody and published. Instruction and protocol was well described. I strongly recommend this antibody to other researcher.
Data di pubblicazione: 2018-08-24
Rated 5 di 5 di da EXCELLENT SENSITIVITY TO SEE VDR ON PROTEIN LEVEL IS VERY TOUGH, BUT USING THE HYPEROSMOLAR BUFFER LYSIS AND THIS VDR ANTIBODY. I GOT THE BAND OF VDR. THIS ANTIBODY IS BETTER THAN ABCAM. THANK YOU
Data di pubblicazione: 2018-04-12
Rated 5 di 5 di da Works well for immunofluorescence and wester blot This antibody works very well at 1:1000 dilution for western blot and at 1:200 dilution for immunofluorescence on mouse calvariae.
Data di pubblicazione: 2018-03-16
Rated 4 di 5 di da Arterial immunohistochemistry VDR antibody is a nuclear antibody, but the cytoplasm is also stained. Look at the pictures provided by manual also have cytoplasmic staining. Do you know how to explain properly?
Data di pubblicazione: 2017-10-16
  • y_2021, m_9, d_20, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_29
  • loc_it_IT, sid_13133, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT