Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VAX2 CRISPR/Cas9 KO Plasmid (h): sc-407255
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VAX2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VAX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VAX2 Antibody (VAX2A8F12): sc-81422
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VAX2 CRISPR/Cas9 KO Plasmid (h) | sc-407255 | 20 µg | $397.00 |
Overview
VAX2 (ventral anterior homeobox 2) encodes a homeobox transcription factor that regulates gene expression programs required for embryonic patterning of the ventral retina and optic stalk. It functions within developmental transcriptional networks that intersect with morphogen signaling, including gradients of Sonic hedgehog (SHH) and retinoic acid, to establish dorsoventral identity and neuroepithelial fate decisions. By controlling spatially restricted transcription in the developing eye, VAX2 helps coordinate retinal cell differentiation, axon guidance, and optic nerve formation. Dysregulated VAX2 expression or altered regulatory circuitry has been associated with congenital ocular malformations and neurodevelopmental phenotypes, making it a useful node for studying human eye development and disease-relevant differentiation pathways.
VAX2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VAX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VAX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VAX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VAX2 protein expression.
This CRISPR knockout system enables efficient generation of VAX2-deficient cell models for investigation of VAX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VAX2 exon(s) critical for VAX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VAX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VAX2 CRISPR/Cas9 KO Plasmid (h) and VAX2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VAX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VAX2 HDR Plasmid (h) and VAX2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VAX2 homology arms to support homology-directed repair at defined VAX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.