Anticorpo ULBP1 (3F1) raccomandato per il rilevamento di ULBP1 di origine human in IP, IF e FCM

Anticorpo ULBP1 (3F1): sc-53131

Anticorpo ULBP1 (3F1) valutazione 3.8 di 5 di 6.
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Schede tecniche

    • Anti-ULBP1 Antibody 3F1 è un monoclonale di topo IgM κ ULBP1 antibody, citato in 5 pubblicazioni, fornito in 200 µg/ml
    • raised against purified human ULBP1
    • raccomandato per il rilevamento di ULBP1 di origine human in IP, IF e FCM
    • also provided azide-free for blocking, sc-53131 L, 200 µg/0.1 ml
    • Contattaci per ricevere un FREE 10 µg sample di ULBP1 (3F1): sc-53131.
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Which secondaries are good for immunofluorescence? Do I need IgM secondaries?

Richiesta da: Kale
Thank you for your question. This mouse monoclonal antibody, ULBP1 (3F1): sc-53131, is an IgM and using an isotype-specific secondary antibody (anti-mouse IgM) is recommended. Alternatively, we also recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to a fluorochrome, such as fluorescein (FITC), m-IgGκ BP-FITC: sc-516140. Other fluorochromes available include: m-IgGκ BP-CFL 488: sc-516176, m-IgGκ BP-CFL 555: sc-516177, or m-IgGκ BP-CFL 594: sc-516178. We have tested and confirmed reactivity of our mouse IgG kappa binding protein with mouse IgM.
Risposta da: Technical Support
Data di pubblicazione: 2017-11-22

I bought the L form of this antibody, sc-53131 L for blocking (neutralization). How long can I store this at in the refridgerator?

Richiesta da: Trav11
The L form of the antibody should be stored at -20°C. Once thawed, we recommend using as soon as possible, although it is usually stable for up to 2 weeks at 4 degrees.
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 2 di 5 di da Very faint signal by FCM This antibody was tested by indirect FCM on HFF cells but does not give a very clean signal, even at dilution 1:50.
Data di pubblicazione: 2018-11-02
Rated 4 di 5 di da Very good antibody I got the sample from santa cruz last month. This work perfectly in the lung fibroblasts that we work with. I am really glad that it worked as I did not find any other better antibody. Good luck...
Data di pubblicazione: 2018-01-17
Rated 5 di 5 di da Good Fluorescent Staining This ULBP1 antibody shows good cytoplasmic staining when used with methanol-fixed HeLa cells in IF. Also works in Flow Cytometry.
Data di pubblicazione: 2017-02-02
Rated 4 di 5 di da IHC(P) publishable data with human HCC tissues IHC(P) publishable data with human HCC tissues - SCBT Publication Review
Data di pubblicazione: 2015-05-02
Rated 4 di 5 di da Clear immunofluorescence cytoplasmic staining Clear immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-02-08
Rated 4 di 5 di da Good indirect FCM data of C1R mock and C1R Good indirect FCM data of C1R mock and C1R-ULBP1 transfectants stained with ULBP1 -SCBT QC
Data di pubblicazione: 2014-09-29
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