Sulpho NHS biotin CAS: 119616-38-5
MF: C14H18N3O8S2Na
MW: 443.42
A water-soluble biotinylation reagent.

Sulpho NHS biotin (CAS 119616-38-5)

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Nomi alternativi: Sulfosuccinimidyl biotin
Applicazione: Sulpho NHS biotin is a water-soluble biotinylation reagent
Numero CAS: 119616-38-5
Peso molecolare: 443.42
Formula molecolare: C14H18N3O8S2Na
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
* Vedere Certificato di Analisi per informazioni sul lotto specifico (incluso il contenuto d'acqua).

Sulpho NHS biotin (Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent used for simple and efficient labeling of antibodies, proteins and primary amine-containing molecules. Biotin is a vitamin which binds with high affinity to avidin and streptavidin proteins. Biotin is small (244 Da), and may be conjugated to proteins without altering their biological activities. NHS-activated biotins couple to primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Sulpho NHS biotin has also been used for labeling of membrane proteins; due to the charge of the sodium sulfoxide group on the succinimidyl ring, it cannot permeate the cell membrane. If the cell remains intact, only primary amines exposed on the surface will be biotinylated. Recommended for use with avidin-conjugated fluorescent molecules: avidin-FITC, sc-2865; avidin-PE, sc-2876; avidin-APC, sc-2898; avidin-PE-Cy7, sc-3769; avidin-APC-Cy7, sc-3780; DY-550-avidin, sc-221567; DY-630-avidin, sc-221572.


Referenze

1. Yamamoto, A.M., et al. 1993. Clin. Exp. Immunol. 92: 381-390. PMID: 7685669
2. Pintado, C.O., et al. 1995. Vet. Immunol. Immunopathol. 44: 359-368. PMID: 7538249
3. Coonrod, S., et al. 1999. Mol. Hum. Reprod. 5: 1027-1033. PMID: 10541564
4. Hewett, P.W. 2001. Int. J. Biochem. Cell Biol. 33: 325-335. PMID: 11312103

Stato fisico :
Solid
Solubilità :
Soluble in water (10 mg/ml), and DMF (50 mg/ml).
CONSERVAZIONE :
Store at 4° C
Indice di rifrazione :
n20D ~1.65 (Predicted)
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
WGK Germania :
3
PubChem CID :
23667738
Numero MDL :
MFCD00078535
SMILES :
C1[[email protected]]2[[email protected]@H](C(S1)CCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)[O-])NC(=O)N2.[Na+]

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