Anticorpo Sprouty 1 (H-2) raccomandato per il rilevamento di Sprouty 1 di origine e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo Sprouty 1 (H-2): sc-365520

Anticorpo Sprouty 1 (H-2) valutazione 5.0 di 5 di 2.
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Schede tecniche

    • Anti-Sprouty 1 Antibody H-2 è un monoclonale di topo IgG1 κ Sprouty 1 antibody, citato in 2 pubblicazioni, fornito in 200 µg/ml
    • raised against amino acids 61-180 mapping within an internal region of Sprouty 1 of human origin
    • Sprouty 1 Antibody (H-2) é raccomandato per il rilevamento di Sprouty 1 di origine human in WB, IP, IF, IHC(P) e ELISA
    • Sprouty 1 Antibody (H-2) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di Sprouty 1 (H-2): sc-365520.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for Sprouty 1 Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Sprouty 1 Antibody (H-2) (see ordering information below).
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Can H-2 monoclonal antibody detect mouse Spry1 in western blot ?? If mice spry1 detect, can you display some data or reference ?

Richiesta da: gogogo
Thank you for your question. We have not yet tested this antibody, Sprouty 1 (H-2): sc-365520, in mouse samples, and so that species is not recommended at this time. We are currently offering a free 10 µg sample of this antibody. If you wish to test it in your mouse samples, please contact our Technical Service Department to request a free sample. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Risposta da: Technical Support
Data di pubblicazione: 2017-08-01

What is the recommended fixation method for immunofluorescence staining with Sprouty 1 (H-2): sc-365520 monoclonal antibody?

Richiesta da: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-27
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Rated 5 di 5 di da Produced great immunofluorescence cyto Produced great immunofluorescence cyto-plasmic staining in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-02-26
Rated 5 di 5 di da Produced positive Western Blot data of Sprouty Produced positive Western Blot data of Sprouty 1 expression in Hep G2 whole cell lysate. -SCBT QC
Data di pubblicazione: 2013-07-13
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