SP Double Nickase Plasmid (h): sc-404996-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SP Double Nickase Plasmid (h) and SP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TFF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SP Antibody (4G7C3): sc-517213
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SP Double Nickase Plasmid (h) | sc-404996-NIC | 20 µg | $410.00 | |||
SP Double Nickase Plasmid (h2) | sc-404996-NIC-2 | 20 µg | $410.00 |
Trefoil factor 2 (TFF2) encodes a secreted, mucin-associated peptide that contributes to gastric and intestinal epithelial protection by promoting restitution, modulating mucus barrier properties, and supporting wound-associated migration. TFF2 is induced during mucosal injury and interacts with epithelial and stromal signaling networks that shape inflammatory responses and tissue remodeling, including pathways linked to cytokine signaling and extracellular matrix dynamics. Dysregulated TFF2 expression has been associated with chronic gastritis, inflammatory bowel conditions, and gastrointestinal tumorigenesis, where altered barrier function and wound-healing programs can influence disease progression. As a marker and mediator of mucosal repair, TFF2 is widely studied in epithelial homeostasis, metaplasia, and microenvironment-driven inflammation models.
SP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TFF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TFF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TFF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TFF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.