SmcX Double Nickase Plasmid (m): sc-423030-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SmcX Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SmcX Double Nickase Plasmid (m) and SmcX Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Kdm5c. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SmcX Antibody (G-10): sc-376255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SmcX Double Nickase Plasmid (m) | sc-423030-NIC | 20 µg | $410.00 | |||
SmcX Double Nickase Plasmid (m2) | sc-423030-NIC-2 | 20 µg | $410.00 |
Kdm5c (SmcX) encodes an X-linked histone lysine demethylase that removes H3K4me2/3 marks to shape transcriptional programs and chromatin accessibility in mouse cells. SmcX participates in epigenetic regulation of neuronal development, synaptic function, and cell fate decisions by modulating promoter and enhancer activity within RNA polymerase II–dependent gene expression pathways. Disruption of Kdm5c alters activity-dependent transcription and is linked to neurodevelopmental phenotypes, providing a mechanistic entry point to study chromatin-driven regulation of cognition and behavior. In addition, Kdm5c-dependent control of differentiation and cell-cycle–associated transcription supports research into context-specific epigenetic remodeling across tissues.
SmcX Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Kdm5c locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Kdm5c. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Kdm5c function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Kdm5c-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.