SHIP-2 Antibody (B-9): sc-515211

SHIP-2 Antibody (B-9) is a mouse monoclonal IgG3 antibody that detects SHIP-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SHIP-2, a homolog of SHIP, plays a crucial role in cellular signaling pathways, particularly in the regulation of the phosphoinositide 3-kinase (PI3K) pathway, which is vital for cell growth and survival. SHIP-2 is predominantly expressed in both hematopoietic and non-hematopoietic cells, including T and B cells, as well as in tissues such as the spleen, thymus, and lung. SHIP-2 expression is notably enhanced in response to stimuli like thyroid-stimulating hormone (TSH) and epidermal growth factor (EGF), indicating involvement in cellular responses to growth factors. SHIP-2 mediates cell cycle arrest in the G1 phase of glioblastoma cells, highlighting potential role as a tumor suppressor. By associating with the Shc protein following B-cell receptor engagement, SHIP-2 contributes to the negative regulation of the PI3K pathway, thereby inhibiting cell proliferation. SHIP-2′s ability to interact with various signaling molecules underscores importance in maintaining cellular homeostasis and potential implications in cancer biology and immune regulation.

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SHIP-2 Antibody (B-9) References:

1. SHIP-2 forms a tetrameric complex with filamin, actin, and GPIb-IX-V: localization of SHIP-2 to the activated platelet actin cytoskeleton.  |  Dyson, JM., et al. 2003. Blood. 102: 940-8. PMID: 12676785
2. SHIP-2 inositol phosphatase is inducibly expressed in human monocytes and serves to regulate Fcgamma receptor-mediated signaling.  |  Pengal, RA., et al. 2003. J Biol Chem. 278: 22657-63. PMID: 12690104
3. The inositol phosphatase SHIP-2 down-regulates FcgammaR-mediated phagocytosis in murine macrophages independently of SHIP-1.  |  Ai, J., et al. 2006. Blood. 107: 813-20. PMID: 16179375
4. Catalytically inactive SHIP2 inhibits proliferation by attenuating PDGF signaling in 3T3-L1 preadipocytes.  |  Artemenko, Y., et al. 2009. J Cell Physiol. 218: 228-36. PMID: 18814181
5. SHIP2 (SH2 domain-containing inositol phosphatase 2) SH2 domain negatively controls SHIP2 monoubiquitination in response to epidermal growth factor.  |  De Schutter, J., et al. 2009. J Biol Chem. 284: 36062-36076. PMID: 19880507
6. PI3K/SHIP2/PTEN pathway in cell polarity and hepatitis C virus pathogenesis.  |  Awad, A. and Gassama-Diagne, A. 2017. World J Hepatol. 9: 18-29. PMID: 28105255
7. Decreased Sp1 Expression Mediates Downregulation of SHIP2 in Gastric Cancer Cells.  |  Ye, Y., et al. 2017. Int J Mol Sci. 18: PMID: 28117748
8. IQGAP2 Inhibits Migration and Invasion of Gastric Cancer Cells via Elevating SHIP2 Phosphatase Activity.  |  Xu, L., et al. 2020. Int J Mol Sci. 21: PMID: 32183047
9. Ebselen enhances insulin sensitivity and decreases oxidative stress by inhibiting SHIP2 and protects from inflammation in diabetic mice.  |  Polianskyte-Prause, Z., et al. 2022. Int J Biol Sci. 18: 1852-1864. PMID: 35342343
10. The Functional Roles of the Src Homology 2 Domain-Containing Inositol 5-Phosphatases SHIP1 and SHIP2 in the Pathogenesis of Human Diseases.  |  Müller, SM. and Jücker, M. 2024. Int J Mol Sci. 25: PMID: 38791291

The preferred SHIP-2 antibody is SHIP-2 Antibody (E-2): sc-166641.