Set1B CRISPR/Cas9 KO Plasmid (m): sc-431532
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Set1B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Set1B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Set1B CRISPR/Cas9 KO Plasmid (m) | sc-431532 | 20 µg | $397.00 |
Overview
Setd1b encodes the Set1B histone lysine methyltransferase, a core component of COMPASS-like chromatin-modifying complexes that catalyze H3K4 methylation at active promoters and regulatory elements. Through modulation of H3K4me1/2/3 states, Set1B helps coordinate transcription initiation, RNA polymerase II activity, and epigenetic maintenance of cell identity programs. Setd1b-dependent chromatin regulation is linked to developmental and neuronal gene expression networks, and altered SETD1B function is associated with neurodevelopmental phenotypes and broader epigenetic dysregulation relevant to disease biology. In mouse systems, Set1B provides a tractable target for dissecting how promoter-associated histone methylation shapes signaling-responsive transcription and genome-wide chromatin landscapes.
Set1B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Setd1b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Setd1b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Setd1b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Set1B protein expression.
This CRISPR knockout system enables efficient generation of Setd1b-deficient cell models for investigation of Set1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Setd1b exon(s) critical for Set1B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Setd1b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Set1B CRISPR/Cas9 KO Plasmid (m) and Set1B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Setd1b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Set1B HDR Plasmid (m) and Set1B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Setd1b homology arms to support homology-directed repair at defined Setd1b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.