SEC23 CRISPR Activation Plasmid (h): sc-403549-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEC23A CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- SEC23A CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by SEC23A CRISPR Activation Plasmid (h) and SEC23A CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the SEC23A transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEC23 CRISPR Activation Plasmid (h) | sc-403549-ACT | 20 µg | $397.00 |
Human SEC23A encodes SEC23, an essential component of the COPII coat that mediates vesicle budding from endoplasmic reticulum exit sites and cargo selection for ER-to-Golgi transport. SEC23 coordinates with SEC24 and the SAR1 GTPase to drive coat assembly, contributing to secretory pathway flux, proteostasis, and proper maturation of extracellular matrix and membrane proteins. Disruption of COPII trafficking can alter glycosylation, ER stress responses, and secretion-dependent signaling networks important in development and tissue homeostasis. Pathogenic SEC23A variation has been linked to craniofacial and skeletal phenotypes, making SEC23A a useful node for studying secretion-related disease mechanisms in human cell models.
SEC23A CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous SEC23A expression without altering the underlying DNA sequence.
SEC23A CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the SEC23A locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the SEC23A transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous SEC23A expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native SEC23A locus and enabling the study of SEC23A-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of SEC23A pathway restoration in tumor cells with silenced or reduced SEC23A expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.