SAS-6 Antibody (G-1): sc-376836

SAS-6 Antibody (G-1) is a mouse monoclonal IgG1 antibody that detects SAS-6 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAS-6, a 657 amino acid protein encoded by the human gene SAS6, plays a crucial role in the formation of centrioles, which are essential for proper cell division and organization of the mitotic spindle. Anti-SAS-6 antibody (G-1) localizes asymmetrically next to the centriole at the onset of procentriole formation, highlighting its importance in the early stages of centriole biogenesis. Notably, SAS-6 levels fluctuate throughout the cell cycle, being degraded during mitosis and accumulating again at the end of the G1 phase, a process regulated by the anaphase-promoting complex targeting SAS-6 for degradation via the 26S proteasome. The presence of a KEN box in the C-terminus of SAS-6 is critical for this degradation process. Furthermore, elevated levels of SAS-6 can lead to the formation of multiple procentrioles adjacent to a single centriole, which underscores its regulatory role in centriole duplication and the potential implications for cellular organization and division. SAS-6 monoclonal antibody (G-1) serves as an invaluable tool for researchers studying centriole dynamics and related cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SAS-6 Antibody (G-1) References:

1. SAS-6 is a cartwheel protein that establishes the 9-fold symmetry of the centriole.  |  Nakazawa, Y., et al. 2007. Curr Biol. 17: 2169-74. PMID: 18082404
2. Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly.  |  Lettman, MM., et al. 2013. Dev Cell. 25: 284-98. PMID: 23673331
3. The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.  |  Cottee, MA., et al. 2015. Elife. 4: e07236. PMID: 26002084
4. Human SAS-6 C-Terminus Nucleates and Promotes Microtubule Assembly in Vitro by Binding to Microtubules.  |  Gupta, H., et al. 2015. Biochemistry. 54: 6413-22. PMID: 26422590
5. De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.  |  Wang, WJ., et al. 2015. Elife. 4: PMID: 26609813
6. High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6.  |  Nievergelt, AP., et al. 2018. Nat Nanotechnol. 13: 696-701. PMID: 29784964
7. Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture.  |  Banterle, N., et al. 2021. Nat Commun. 12: 6180. PMID: 34702818
8. Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel.  |  Kantsadi, AL., et al. 2022. Structure. 30: 671-684.e5. PMID: 35240058
9. The chromatin remodeling protein CHD-1 and the EFL-1/DPL-1 transcription factor cooperatively down regulate CDK-2 to control SAS-6 levels and centriole number.  |  Iyer, J., et al. 2022. PLoS Genet. 18: e1009799. PMID: 35377871
10. Grand canonical Brownian dynamics simulations of adsorption and self-assembly of SAS-6 rings on a surface.  |  Gomez Melo, S., et al. 2023. J Chem Phys. 158: 085102. PMID: 36859084

The preferred SAS-6 antibody is SAS-6 Antibody (91.390.21): sc-81431.