Anticorpo RNase L (2E9.2G5) raccomandato per il rilevamento di RNase L di origine human in WB, IP, IF e IHC(P)

Anticorpo RNase L (2E9.2G5): sc-23955

Anticorpo RNase L (2E9.2G5) valutazione 5.0 di 5 di 2.
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Schede tecniche

    • RNase L Antibody 2E9.2G5 è un monoclonale di topo IgG1 κ, citato in 2 pubblicazioni, fornito in 200 µg/ml
    • raised against purified recombinant human RNase L
    • raccomandato per il rilevamento di RNase L di origine human in WB, IP, IF e IHC(P)
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RNase L Antibody (2E9.2G5) for WB and IHC(P) applications. These reagents are now offered in bundles with RNase L Antibody (2E9.2G5) (see ordering information below).
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Can RNase L (2E9.2G5): sc-23955 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Richiesta da: jenniferc
Thank you for your question. Yes, RNase L (2E9.2G5): sc-23955 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-25

Dear Santa Cruz,  I use this antibody to detect endogenous RNase L. Activated RNase L is dimerized and it can show two bands but in case of this antibody is it possible to see difference between activated and non activated RNase L? Thank you!

Richiesta da: Idee
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, RNase L (2E9.2G5): sc-23955, has not been determined. Thus, it would have to be determined experimentally if the antibody can distinguish between activated and non-activated RNase L. This experiment has not been performed by Santa Cruz Biotechnology.
Risposta da: Technical Support
Data di pubblicazione: 2020-10-13
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Rated 5 di 5 di da Positive results in western blot analysis Positive results in western blot analysis of RNase L expression in DU 145 and PC-3 whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-04-09
Rated 5 di 5 di da Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Data di pubblicazione: 2015-01-27
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