Anticorpo RAGE (D-5) raccomandato per il rilevamento di RAGE di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo RAGE (D-5): sc-74535

Anticorpo RAGE (D-5) valutazione 4.3 di 5 di 4.
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Schede tecniche

    • RAGE Antibody D-5 è un monoclonale di topo IgG3 κ, citato in 3 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 1-300 di RAGE di origine human
    • raccomandato per il rilevamento di RAGE di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Vedere RAGE (A-9): sc-365154 for RAGE per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RAGE Antibody (D-5). This work is in progress.
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Can RAGE (D-5): sc-74535 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Richiesta da: Cweed
Thank you for your question. Yes, RAGE (D-5): sc-74535 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-25
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Rated 4 di 5 di da Various publishable data in WB using human Various publishable data in WB using human HEK293 cells. -SCBT Publication Review
Data di pubblicazione: 2015-04-25
Rated 4 di 5 di da Great band seen in WB using human recombinant Great band seen in WB using human recombinant RAGE protein. -SCBT QC
Data di pubblicazione: 2015-04-16
Rated 5 di 5 di da Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Data di pubblicazione: 2015-01-29
Rated 4 di 5 di da Publishable data in WB using rat liver and aorta Publishable data in WB using rat liver and aorta. -SCBT Publication Review
Data di pubblicazione: 2013-04-13
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