Puromycin dihydrochloride CAS: 58-58-2
MF: C22H29N7O5•2HCl
MW: 544.43

Puromycin dihydrochloride (CAS 58-58-2)

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Puromycin dihydrochloride is rated 4.93 out of 5 by 14.
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Nomi alternativi: Puromycin dihydrochloride is also known as Stylomycin hydrochloride
Applicazione: Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene
Numero CAS: 58-58-2
Purezza: ≥98%
Peso molecolare: 544.43
Formula molecolare: C22H29N7O52HCl
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
* Vedere Certificato di Analisi per informazioni sul lotto specifico (incluso il contenuto d'acqua).
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Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.
Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase.


Referenze

1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187

Utilizzo :
For the selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles, proceed with puromycin selection as follows:

NOTE: The working puromycin concentration for selection in mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell line (see below). Use the lowest concentration that kills 100% of non-transfected cells in 3-5 days from the start of puromycin selection.


•48 hours post-transfection, aspirate the medium and replace with fresh medium containing puromycin at the appropriate concentration.

•Approximately every 2-3 days, aspirate and replace with freshly prepared selective media.

•Monitor the cells daily. Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days.

•Assay transfected cells.

NOTE: Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of puromycin should be titrated.
Titrating puromycin for selecting transfected cell lines:

•Plate 2 x 105 cells in each well of a 6-well plate containing 3 ml of the appropriate complete medium plus increasing concentrations of puromycin (i.e., 0, 1.0, 2.5, 5.0, 7.5, and 10.0 µg/ml)

•Replace with fresh selective medium after 2 days to remove dead cells.

•Examine the wells for viable cells every two days.

•Monitor the cells daily and observe the percentage of surviving cells. Optimum effectiveness should be reached in 1-4 days.

•The minimum antibiotic concentration to use is the lowest concentration that kills 100% of the cells in 3-5 days from the start of puromycin selection.
Aspetto :
Powder
Stato fisico :
Solid
Derivato da :
Streptomyces alboniger
Solubilità :
Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 7.2 (~10 mg/ml).
CONSERVAZIONE :
Desiccate at -20° C
Punto di scioglimento :
178-180° C
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
WGK Germania :
3
RTECS :
AU7355000
PubChem CID :
Indice Merck :
14: 7943
Numero MDL :
MFCD00012691
Numero EC :
200-387-8
Registro Beilstein :
3853613
SMILES :
CN(C)C1=NC=NC2=C1N=CN2[[email protected]]3[[email protected]@H]([[email protected]@H]([[email protected]](O3)CO)NC(=O)[[email protected]@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

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Puromycin dihydrochloride (CAS 58-58-2)  Citazioni prodotti

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PMID: # 33436530  Li, F.|Zhang, J.|Chen, A.|Liao, R.|Duan, Y.|Xu, Y.|Tao, L.| et al. 2021. Aging (Albany NY). 13: 262-278.

PMID: # 33756106  Villa, E.|Sahu, U.|O'Hara, BP.|Ali, ES.|Helmin, KA.|Asara, JM.|Gao, P.|Singer, BD.|Ben-Sahra, I.| et al. 2021. Mol Cell.

PMID: # 33802790  Naffa, R.|Padányi, R.|Ignácz, A.|Hegyi, Z.|Jezsó, B.|Tóth, S.|Varga, K.|Homolya, L.|Hegedűs, L.|Schlett, K.|Enyedi, A.| et al. 2021. Cancers (Basel). 13:

PMID: # 33846462  Hamar, J.|Kültz, D.| et al. 2021. Sci Rep. 11: 7854.

PMID: # 34007175  Zhang, L.|Wang, M.|Zhu, Z.|Ding, C.|Chen, S.|Wu, H.|Yang, Y.|Che, F.|Li, Q.|Li, H.| et al. 2021. Int J Nanomedicine. 16: 3217-3240.

PMID: # 33440192  Sauter, MM.|Brandt, CR.| et al. 2021. Exp Eye Res. 108436.

PMID: # 33692342  Zhang, G.|Li, X.|Wu, L.|Qin, YX.| et al. 2021. Bone Res. 9: 16.

PMID: # 33671709  Pietrzak, J. et al. 2021. Pharmaceuticals (Basel, Switzerland). 14:

PMID: # 34088927  Lee, JC.|Yotis, DM.|Lee, JY.|Sarabusky, MA.|Shrum, B.|Champagne, A.|Ismail, OZ.|Tutunea-Fatan, E.|Leong, HS.|Gunaratnam, L.| et al. 2021. Sci Rep. 11: 11840.

PMID: # 33547545  Li, H.|Weng, Y.|Lai, L.|Lei, H.|Xu, S.|Zhang, Y.|Li, L.| et al. 2021. Mol Cell Biochem. 476: 2125-2134.

Ciatazioni 1 a10 di 269 totali
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