PRDM5 CRISPR/Cas9 KO Plasmid (m): sc-427796
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRDM5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRDM5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRDM5 Antibody (A-12): sc-376277
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRDM5 CRISPR/Cas9 KO Plasmid (m) | sc-427796 | 20 µg | $397.00 |
Overview
Prdm5 encodes PRDM5, a PR/SET domain-containing transcriptional regulator that modulates gene expression programs involved in differentiation, extracellular matrix organization, and cell-cycle control. PRDM5 has been linked to epigenetic regulation through interactions with chromatin-modifying machinery, influencing transcriptional repression/activation at target loci. In mouse systems, Prdm5 activity has been associated with connective tissue and bone-related developmental processes, reflecting its role in regulating matrix and structural gene networks. Altered PRDM5 function has been studied in the context of dysregulated growth control and epigenetic imbalance relevant to tumor biology and fibrotic-like transcriptional states.
PRDM5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prdm5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prdm5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prdm5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRDM5 protein expression.
This CRISPR knockout system enables efficient generation of Prdm5-deficient cell models for investigation of PRDM5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prdm5 exon(s) critical for PRDM5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prdm5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRDM5 CRISPR/Cas9 KO Plasmid (m) and PRDM5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prdm5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRDM5 HDR Plasmid (m) and PRDM5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prdm5 homology arms to support homology-directed repair at defined Prdm5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.