Anticorpo PP2B-A alpha (D-9) raccomandato per il rilevamento di PP2B-Aα di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo PP2B-Aα (D-9): sc-17808

Anticorpo PP2B-Aα (D-9) valutazione 4.6 di 5 di 12.
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Schede tecniche

    • PP2B-A alpha Antibody D-9 è un monoclonale di topo IgG2b κ PP2B-Aα antibody, citato in 13 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 312-521 di protein phosphatase 2B catalytic Aα subunit (PP2B-Aα) di origine human
    • PP2B-A alpha Antibody (D-9) é raccomandato per il rilevamento di PP2B-Aα di origine mouse, rat e human in WB, IP, IF e ELISA; anche reattivo con ulteriori specie, incluse e equine and canine
    • PP2B-A alpha Antibody (D-9) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di PP2B-Aα (D-9): sc-17808.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PP2B-Aα Antibody (D-9) for WB applications. These reagents are now offered in bundles with PP2B-Aα Antibody (D-9) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with PP2B-Aα (D-9): sc-17808 monoclonal antibody?

Richiesta da: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da マウス脳凍結切片の免疫染色にワーク マウス脳凍結切片を抗原不活化(クエン酸バッファー:MW処理5min)して200倍希釈でよく染まります。
Data di pubblicazione: 2020-10-02
Rated 4 di 5 di da It works well The sample of this product works well. I got a thin bind of my protein sample from osteoclasts.
Data di pubblicazione: 2018-03-27
Rated 5 di 5 di da Works for IP Works for IP. Had previously tried calcineurin antibody from CST which didn't work.
Data di pubblicazione: 2017-12-26
Rated 4 di 5 di da Good clean bands, sadly several products Sadly, there were more than one products turning up for western blots (1:1000) using this, but otherwise, nice clean bands where I needed them.
Data di pubblicazione: 2017-11-07
Rated 5 di 5 di da Calcineurin antibody Mouse anti-CNalpha antibody (sc-17808) works great on immunoblotting at 1:1000 in 2.5 % milk powder. It can be reused and recognizes a 60 kDa band in all blood cancer cells tested in my lab including J77 cells.
Data di pubblicazione: 2017-08-24
Rated 3 di 5 di da Work for WB Use siPP2B MEF cell sample, Ab diluted to 1:1000, got the single and clear band at the right site.
Data di pubblicazione: 2017-08-17
Rated 5 di 5 di da Excellent antibody for Western blotting. This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects an appropriately sized ~59 kDa target protein in immunoblots.
Data di pubblicazione: 2016-10-13
Rated 5 di 5 di da Produced excellent immunofluorescence cytoplasmic Produced excellent immunofluorescence cytoplasmic staining in methanol-fixed EOC 20 cells. -SCBT QC
Data di pubblicazione: 2015-05-04
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