Anticorpo PP2A-C alpha / beta (1D6) raccomandato per il rilevamento di PP2A-Cα/β di origine mouse, rat, human, bovine, porcine, canine e bacterial in WB, IP, IF and IHC(P)

Anticorpo PP2A-Cα/β (1D6): sc-80665

Anticorpo PP2A-Cα/β (1D6) valutazione 4.9 di 5 di 15.
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Schede tecniche

    • PP2A-C alpha / beta Antibody 1D6 è un monoclonale di topo IgG2b κ, citato in 31 pubblicazioni, fornito in 100 µg/ml
    • raised against amino acids 295-309 of PP2A-Cα/β of human PP2A
    • PP2A-C alpha / beta Antibody (1D6) é raccomandato per il rilevamento di PP2A-Cα/β di origine mouse, rat, human, bovine, porcine, canine e bacterial in WB, IP, IF and IHC(P); da demetilare, trattare con 100 mM NaOH in ghiaccio
    • PP2A-C alpha / beta Antibody (1D6) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di PP2A-Cα/β (1D6): sc-80665.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PP2A-Cα/β Antibody (1D6) for WB and IHC(P) applications. These reagents are now offered in bundles with PP2A-Cα/β Antibody (1D6) (see ordering information below).
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Can you recommend a positive control to use with PP2A-Cα/β (1D6): sc-80665 monoclonal antibody for Western blot?

Richiesta da: jenni
We recommend U-698-M (sc-364799), NIH/3T3 (sc-2210) or 3T3-L1 (sc-2243) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of PP2A-Cα/β in these cell lines.
Risposta da: Technical Support 7
Data di pubblicazione: 2017-01-17
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Rated 4 di 5 di da Nice and single band (WB) This antibody well recognizes the human PP2A-C in MDA-MB-231 cells around 36 kDa. Ab incubation: 1:1000 in 5% milk in TBST for 2h at RT.
Data di pubblicazione: 2021-04-30
Rated 5 di 5 di da this antibody works well This has worked very well in WB in HK2 cell lysates and mice kidney tissue
Data di pubblicazione: 2017-11-20
Rated 5 di 5 di da tested in HCC cell lines This antibody work excellent in western blotting. I could get clear and stable single stripe in two different hcc cell lines.
Data di pubblicazione: 2017-08-18
Rated 5 di 5 di da Great performance in WB Checked with siPP2A-MEF cell samples, and first dilute to 1:1000, too much. Then further dilute to 1:5000, even 1:10000, still work for getting clear results.
Data di pubblicazione: 2017-08-17
Rated 5 di 5 di da Excellent This antibody is very good for western blot and shows strong signal in HepG2 cells.I used at 1:1000 dilution.
Data di pubblicazione: 2017-07-14
Rated 5 di 5 di da Great antibody We recently received a sample of PP2A-Cα/β (1D6) antibody and evaluated it's performance and specificity by Western Blot analysis using two different whole cell lysates from HEK 293 and 4 dpf zebrafish larvae (dpf, days post fertilization). There was a single band corresponding to PP2A-C (~36kDa) in both cases.
Data di pubblicazione: 2017-06-30
Rated 5 di 5 di da Great antibody! I used this antibody for WB ,the protein was extracted from h9c2,the band is clear and special!
Data di pubblicazione: 2017-04-10
Rated 5 di 5 di da screening my target protien I wanted to use this PP2A-C alpha / beta antibody to get my experiment more completed.
Data di pubblicazione: 2017-03-24
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