Pepsin 1:10000 is a proteolytic enzyme found in the digestive system. Non-specific background staining in bronchial tissue can be reduced after Pepsin 1:10000 digestion. Pepsin 1:10000 is commonly used to prepare antibody Fab fragments. For example, Pepsin 1:10000 can be used to prepare the F(ab)2 fragment from IgG. Pepsin 1:10000, in conjunction with other enzymes, can show the effects of fixation and enzymatic digestion during immunohistochemical assaying.
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