Anticorpo PDGF Receptor beta/PDGFR-beta (D-6) raccomandato per il rilevamento di PDGF receptor type β di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo PDGF Receptor beta/PDGFR-β (D-6): sc-374573

Anticorpo PDGF Receptor beta/PDGFR-β (D-6) valutazione 4.0 di 5 di 14.
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Schede tecniche

    • Anti-PDGF Receptor beta/PDGFR-beta Antibody D-6 è un monoclonale di topo IgG2a κ PDGF Receptor beta/PDGFR-β antibody, citato in 16 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 958-1106 di PDGFR-β di origine human
    • PDGF Receptor beta/PDGFR-beta Antibody (D-6) é raccomandato per il rilevamento di PDGF receptor type β di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • PDGF Receptor beta/PDGFR-beta Antibody (D-6) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di PDGF Receptor beta/PDGFR-β (D-6): sc-374573.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PDGF Receptor beta/PDGFR-β Antibody (D-6) for WB and IHC(P) applications. These reagents are now offered in bundles with PDGF Receptor beta/PDGFR-β Antibody (D-6) (see ordering information below).
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Does this antibody require a cell permeabilization step before incubation?

Richiesta da: w123
Thank you for your question. Cell permeabilization may be necessary for flow cytometry or IHC applications. You can find our recommended protocols for all applications by following this link: https://www.scbt.com/scbt/resources/protocols
Risposta da: SCBT Heidelberg Support
Data di pubblicazione: 2021-07-23

Do you have any recommendations of how to use PDGFRB D-6: sc-374573 in immunohistochemistry? We have tried it with Citrate Buffer and the ABC/DAB kits and it works well, although in some areas the staining looks weak. Any specific products needed?

Richiesta da: Sabine79
Thank you for your question. A link to our recommended protocol for use with this PDGFR-β Antibody (D-6): sc-374573 is provided below. We recommend using sodium citrate buffer (pH 6) with heat treatment for antigen retrieval. And we recommend using the primary at a starting dilution of 1:50. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-11-17

What blocking solution should I use with PDGFR-β (D-6): sc-374573 monoclonal antibody for Western blot?

Richiesta da: jenniferc
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Santa Cruz TS
Data di pubblicazione: 2017-01-31
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Rated 5 di 5 di da Good in WB The quality of this antibody is good in western blotting. I get clear bands by using 1:1000.
Data di pubblicazione: 2021-07-21
Rated 4 di 5 di da Funziona bene in immunofluorescenza Ho provato questo anticorpo in immunofluoresenza (1:100) su spinal cord di topo (fissato e criostatato) e funziona molto bene! Marcatura specifica e poco fondo
Data di pubblicazione: 2020-10-05
Rated 5 di 5 di da Great for IF-P I used the sample sized antibody of sc-374573, it works great for IF-P on mice brain tissue. strongly recommend this one.
Data di pubblicazione: 2019-12-03
Rated 5 di 5 di da Good specificity and signal strength Works well to detect PDGFR in human pulmonary alveolar epithelial cells.
Data di pubblicazione: 2019-03-29
Rated 1 di 5 di da Did not work for WB analysis Tried at a 1:500 dilution in human and canine bladder cancer cells but detected no signal.
Data di pubblicazione: 2018-06-19
Rated 5 di 5 di da worked great This antibody works great for WB,We got strong signal band at 190kDa. The dilution is 1:300,which is best for mouse kidney tissue.
Data di pubblicazione: 2018-05-26
Rated 1 di 5 di da Not valid for IF on rat frozen liver sections Rat liver frozen sections were subjected to immunofluorescence using PDGFR-β (D-6) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. However, a positive signal was not clear on each slide.
Data di pubblicazione: 2018-02-28
Rated 2 di 5 di da Mouse brain - thalamus, unidentifiable structure I did get specific labeling with 1:200 and 1:100 concentrations, but since I am looking for an antibody that labels pericytes, the specific labeling was not located in the desired structure. I don't know much about glial cells/mural cells etc, so I don't dare to say what structure wás labelled, but I saw very little coll IV-labelled capillaries (<10um) with PDGFR-b labelled structures around it (which would be pericytes). So for me it is not a disirable antibody. As far as I know, PDGFR-b should label pericytes, so with my current knowledge I wouldn't say that I'm content with the result.
Data di pubblicazione: 2017-12-21
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