Anticorpo pan 14-3-3 (B-8) raccomandato per il rilevamento di pan 14-3-3 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo pan 14-3-3 (B-8): sc-133233

Anticorpo pan 14-3-3 (B-8) valutazione 4.7 di 5 di 12.
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Schede tecniche

    • Anti-pan 14-3-3 Antibody B-8 è un monoclonale di topo IgG1 κ pan 14-3-3 antibody, citato in 6 pubblicazioni, fornito in 200 µg/ml
    • raised against amino acids 1-246 representing full length 14-3-3 β of human origin
    • pan 14-3-3 Antibody (B-8) é raccomandato per il rilevamento di pan 14-3-3 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • pan 14-3-3 Antibody (B-8) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di pan 14-3-3 (B-8): sc-133233.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pan 14-3-3 Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with pan 14-3-3 Antibody (B-8) (see ordering information below).
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What blocking solution should I use with pan 14-3-3 (B-8): sc-133233 monoclonal antibody for Western blot?

Richiesta da: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support 15
Data di pubblicazione: 2017-02-09
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Rated 4 di 5 di da Good A band of predicted size was detected in WB (HEK293 and mouse brain lysate).
Data di pubblicazione: 2020-06-26
Rated 5 di 5 di da Worked great for human macorphages Worked great for human macrophages at 1:100 dilution.
Data di pubblicazione: 2019-12-04
Rated 5 di 5 di da Great signal to noise by western Work on human (293t) cell, and mouse/rat brain lysate. Nice bright band a bit above 25 kd (brighter ladder band-lane1). Shown, 25ug of rat brain lysate (lane2).
Data di pubblicazione: 2017-05-29
Rated 5 di 5 di da Strong specific band by western blot This antibody works great on western blots using mouse, rat, or human protein samples. Nice, bright, specific band a little over 25 kDa.
Data di pubblicazione: 2017-05-10
Rated 3 di 5 di da worked against only beta myc-14-3-3 b, g, e, z were overexpressed. however only beta was detected. endogenous in 293 cells also detected. but I guess it is beta. 1/1000 dil in 2% BSA TBST, overnight rxn.
Data di pubblicazione: 2017-03-22
Rated 5 di 5 di da Works well Works well for WB. Tested at 1:1000. Happy with the antibody.
Data di pubblicazione: 2017-03-06
Rated 5 di 5 di da Great detection. This antibody will provide great pan 14-3-3 detect in mouse, rat, or human samples. It has great staining data and provides clear and specific WB.
Data di pubblicazione: 2017-01-25
Rated 5 di 5 di da Excellent for WB Received a sample for this antibody. Tried it on MEF and HEK-293. Worked very nicely at 1:1000 dilution. Single band detected and strong signal.
Data di pubblicazione: 2017-01-04
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