PACAP CRISPR/Cas9 KO Plasmid (m): sc-419000
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PACAP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PACAP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PACAP Antibody (F-2): sc-166180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PACAP CRISPR/Cas9 KO Plasmid (m) | sc-419000 | 20 µg | $397.00 |
Overview
Adcyap1 encodes pituitary adenylate cyclase-activating polypeptide (PACAP), a conserved neuropeptide that signals primarily through PAC1 (ADCYAP1R1) and related class B GPCRs to regulate cAMP/PKA, MAPK/ERK, and intracellular Ca²⁺ pathways. In mouse tissues, PACAP modulates neurotransmission, neurodevelopment, neuroendocrine secretion, and cellular stress responses, influencing synaptic plasticity, circadian and hypothalamic homeostasis, and autonomic regulation. PACAP signaling intersects with inflammatory and metabolic programs by shaping cytokine release and cell survival signaling in neural and peripheral compartments. Dysregulated ADCYAP1/PACAP axis has been linked in the literature to stress-related neurobiology, pain processing, and neuroinflammatory mechanisms, supporting mechanistic studies in nervous system and immune-relevant contexts.
PACAP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adcyap1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adcyap1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adcyap1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PACAP protein expression.
This CRISPR knockout system enables efficient generation of Adcyap1-deficient cell models for investigation of PACAP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adcyap1 exon(s) critical for PACAP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adcyap1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PACAP CRISPR/Cas9 KO Plasmid (m) and PACAP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adcyap1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PACAP HDR Plasmid (m) and PACAP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adcyap1 homology arms to support homology-directed repair at defined Adcyap1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.