p53 Antibody (Bp53-12): sc-263
- p53 Antibody (Bp53-12) is a mouse monoclonal IgG2a κ p53 antibody, cited in 203 publications, provided at 200 µg/ml
- raised against the N-terminus of p53 of human origin
- p53 Antibody (Bp53-12) is recommended for detection of all forms of p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-p53 Antibody (Bp53-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p53 Antibody (Bp53-12) for WB and IHC(P) applications. These reagents are now offered in bundles with p53 Antibody (Bp53-12) (see ordering information below).
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p53 Antibody (Bp53-12) is an IgG2a kappa mouse monoclonal p53 antibody (also designated p53 antibody or TP53 antibody) that detects all forms of p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. p53 Antibody (Bp53-12) is available as the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p53 Antibody (Bp53-12) | sc-263 | 200 µg/ml | $322.00 | |||
p53 Antibody (Bp53-12): m-IgG Fc BP-HRP Bundle | sc-528104 | 200 µg Ab; 10 µg BP | $361.00 | |||
p53 Antibody (Bp53-12): m-IgGκ BP-HRP Bundle | sc-520402 | 200 µg Ab, 40 µg BP | $361.00 | |||
p53 Antibody (Bp53-12): m-IgG2a BP-HRP Bundle | sc-547025 | 200 µg Ab; 10 µg BP | $361.00 | |||
p53 Antibody (Bp53-12) AC | sc-263 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p53 Antibody (Bp53-12) HRP | sc-263 HRP | 200 µg/ml | $322.00 | |||
p53 Antibody (Bp53-12) FITC | sc-263 FITC | 200 µg/ml | $336.00 | |||
p53 Antibody (Bp53-12) PE | sc-263 PE | 200 µg/ml | $349.00 | |||
p53 Antibody (Bp53-12) Alexa Fluor® 488 | sc-263 AF488 | 200 µg/ml | $364.00 | |||
p53 Antibody (Bp53-12) Alexa Fluor® 546 | sc-263 AF546 | 200 µg/ml | $364.00 | |||
p53 Antibody (Bp53-12) Alexa Fluor® 594 | sc-263 AF594 | 200 µg/ml | $364.00 | |||
p53 Antibody (Bp53-12) Alexa Fluor® 647 | sc-263 AF647 | 200 µg/ml | $364.00 | |||
p53 Antibody (Bp53-12) Alexa Fluor® 680 | sc-263 AF680 | 200 µg/ml | $364.00 | |||
p53 Antibody (Bp53-12) Alexa Fluor® 790 | sc-263 AF790 | 200 µg/ml | $364.00 |