CDKN2B/p15 INK4B Antibody (D-12): sc-271791

p15 INK4B Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody engineered to recognize human p15 INK4B protein, also known as CDKN2B, MTS2, TP15, and CDK4I. p15 INK4B monoclonal antibody (D-12) targets a precise epitope located between amino acids 103-137 at the C-terminus of p15 INK4B protein, ensuring robust and selective binding in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p15 INK4B protein is primarily localized in the nucleus of the cell, where p15 INK4B plays a crucial role in regulating the cell cycle by inhibiting cyclin-dependent kinases Cdk4 and Cdk6. This inhibition is vital for controlling the transition from the G1 phase to the S phase, thereby maintaining proper cell cycle checkpoints and preventing uncontrolled cell proliferation, which is a key factor in tumor development. p15 INK4B functions as a tumor suppressor and acts as an effector in TGFβ-mediated cell cycle arrest, with p15 INK4B expression being significantly upregulated in response to TGFβ treatment in human keratinocytes. Anti-p15 INK4B antibody (D-12) enables researchers to effectively investigate the regulatory mechanisms of cell cycle control and the role of p15 INK4B in cancer biology, making anti-p15 INK4B antibody (D-12) an indispensable tool for studies focused on cell proliferation and tumor suppression.

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CDKN2B/p15 INK4B Antibody (D-12) References:

1. Methylation of the p15(INK4B) gene in myelodysplastic syndrome: it can be detected early at diagnosis or during disease progression and is highly associated with leukaemic transformation.  |  Tien, HF., et al. 2001. Br J Haematol. 112: 148-54. PMID: 11167795
2. Hypermethylation of the p15(INK4B) gene in acute leukemia and myelodysplastic syndromes.  |  Chen, H. and Wu, S. 2002. Chin Med J (Engl). 115: 987-90. PMID: 12150726
3. Anti-epidermal growth factor receptor monoclonal antibody 225 upregulates p27(KIP1) and p15(INK4B) and induces G1 arrest in oral squamous carcinoma cell lines.  |  Kiyota, A., et al. 2002. Oncology. 63: 92-8. PMID: 12187077
4. Methylation of p15(INK4b) and E-cadherin genes is independently correlated with poor prognosis in acute myeloid leukemia.  |  Shimamoto, T., et al. 2005. Leuk Res. 29: 653-9. PMID: 15863205
5. Differential effect of epigenetic alterations and genomic deletions of CDK inhibitors [p16(INK4a), p15(INK4b), p14(ARF)] in mantle cell lymphoma.  |  Hutter, G., et al. 2006. Genes Chromosomes Cancer. 45: 203-10. PMID: 16258956
6. Histone deacetylase 3 represses p15(INK4b) and p21(WAF1/cip1) transcription by interacting with Sp1.  |  Huang, W., et al. 2006. Biochem Biophys Res Commun. 339: 165-71. PMID: 16298343
7. Regulation of the INK4b-ARF-INK4a tumour suppressor locus: all for one or one for all.  |  Gil, J. and Peters, G. 2006. Nat Rev Mol Cell Biol. 7: 667-77. PMID: 16921403
8. p15(INK4b) plays a crucial role in murine lymphoid development and tumorigenesis.  |  Osei-Sarfo, K., et al. 2012. Carcinogenesis. 33: 708-13. PMID: 22227036
9. Joint Effects Between CDKN2B/P15 Methylation and Environmental Factors on the Susceptibility to Gastric Cancer.  |  Zhou, H., et al. 2023. Dig Dis Sci. 68: 3009-3017. PMID: 36961670
10. p15INK4B is a potential effector of TGF-beta-induced cell cycle arrest.  |  Hannon, GJ. and Beach, D. 1994. Nature. 371: 257-61. PMID: 8078588
11. A cell cycle regulator potentially involved in genesis of many tumor types.  |  Kamb, A., et al. 1994. Science. 264: 436-40. PMID: 8153634
12. The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2.  |  Reynisdóttir, I. and Massagué, J. 1997. Genes Dev. 11: 492-503. PMID: 9042862

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CDKN2B/p15 INK4B Antibody (D-12) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-CDKN2B/p15 INK4B Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CDKN2B/p15 INK4B Antibody (D-12) Specifications

Alternate Product Name: p15 INK4B

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 103-137

Epitope Target: p15 INK4B

Epitope Species: human

Product Reactivity/Detection: p15 INK4B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for CDKN2B/p15 INK4B Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred CDKN2B/CDKN2A/p16 antibody is CDKN2B/CDKN2A/p16 Antibody (C-7): sc-377412.