Anticorpo p-Stat3 (9E12) raccomandato per il rilevamento di Tyr 705 phosphorylated Stat3 di origine mouse, rat, human e canine in WB, IP e IF

Anticorpo p-Stat3 (9E12): sc-81523

Anticorpo p-Stat3 (9E12) valutazione 4.0 di 5 di 5.
  • y_2021, m_9, d_23, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_it_IT, sid_81523, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 7ms
  • REVIEWS, PRODUCT
loading
5
3
4
1
3
0
2
0
1
1

Schede tecniche

    • p-Stat3 Antibody 9E12 è un monoclonale di topo IgG1, citato in 19 pubblicazioni, fornito in 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to the region surrounding p-Tyr 705 of Stat3 of human origin
    • raccomandato per il rilevamento di Tyr 705 phosphorylated Stat3 di origine mouse, rat, human e canine in WB, IP e IF
    • Vedere p-Stat3 (B-7): sc-8059 for p-Stat3 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • Contattaci per ricevere un FREE 10 µg sample di p-Stat3 (9E12): sc-81523.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-Stat3 Antibody (9E12) for WB applications. These reagents are now offered in bundles with p-Stat3 Antibody (9E12) (see ordering information below).
loading

I have read few people mentioned it worked very well for western. Could you tell me the dilution and is it in milk or 5%BSA incubation? Thanks

Richiesta da: mkunni
Thank you for your question. We recommend a starting dilution of 1:200 for Western blotting (range 1:100-1:1,000). We recommend using either 5% nonfat milk + TBST, or a mixture of 1% nonfat milk + 1% BSA in TBST for blocking and incubating both primary and secondary antibodies.
Risposta da: Technical Support
Data di pubblicazione: 2019-06-12

For Western Blot, is it recommended to use denatured or non-denatured conditions with p-Stat3 (9E12): sc-81523 antibody?

Richiesta da: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-27
  • y_2021, m_9, d_23, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_it_IT, sid_81523, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 6ms
  • QUESTIONS, PRODUCT
Rated 1 di 5 di da No detección de banda No se detectó ninguna banda. Se probó el anticuerpo con diferentes cantidades (hasta 100ug) de proteínas extraídas de muestras de tejido.
Data di pubblicazione: 2018-01-03
Rated 5 di 5 di da This product works great in WB This antibody worked really well for us in our Western, thought it was very stable.
Data di pubblicazione: 2017-05-07
Rated 5 di 5 di da Nice results Nice and specific Western blot results in various samples with p-Stat3 Antibody (9E12)
Data di pubblicazione: 2017-01-27
Rated 4 di 5 di da Works well for Western blot Works well for Western blot, strong band detected in IFN- -treated SK-MEL-28 whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-03-12
Rated 5 di 5 di da Great antibody for Western Great antibody for Western, strong band detected in IFN treated HeLa whole cell lysate. -SCBT QC
Data di pubblicazione: 2014-04-14
  • y_2021, m_9, d_23, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_it_IT, sid_81523, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT