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p-Ribosomal Protein S6 Antibody (24.Ser 240) - Western Blotting - Image 159484 — Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 Antibody (24.Ser 240): sc-293143 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).

p-Ribosomal Protein S6 Antibody (24.Ser 240) - Western Blotting - Image 380912 — p-Ribosomal Protein S6 Antibody (24.Ser 240): sc-293143. Western blot analysis of Ribosomal Protein S6 phosphorylation in MDA-MB-435S (A) and NIH/3T3 (B) whole cell lysates.

p-Ribosomal Protein S6 Antibody (24.Ser 240) - Western Blotting - Image 357441 — p-Ribosomal Protein S6 Antibody (24.Ser 240): sc-293143. Western blot analysis of Ribosomal Protein S6 phosphorylation in MDA-MB-435S whole cell lysate.

Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 Antibody (24.Ser 240): sc-293143 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).

p-Ribosomal Protein S6 Antibody (24.Ser 240): sc-293143

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p-Ribosomal Protein S6 Antibody (24.Ser 240) is a mouse monoclonal IgG₁, cited in 5 publications, provided at 200 µg/ml
raised against a short amino acid sequence containing phosphorylated Ser 240 of Ribosomal Protein S6 of human origin
recommended for detection of Ser 240 phosphorylated Ribosomal Protein S6 of mouse, rat, human origin by WB, IP and ELISA
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Ribosomal Protein S6 Antibody (24.Ser 240) for WB applications. These reagents are now offered in bundles with p-Ribosomal Protein S6 Antibody (24.Ser 240) (see ordering information below).

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Additional Ribosomal Protein Antibodies including Ribosomal Protein L11, Ribosomal Protein L13, Ribosomal Protein L14, Ribosomal Protein L17, Ribosomal Protein L18, Ribosomal Protein L19, Ribosomal Protein L21, Ribosomal Protein L22, Ribosomal Protein L23a, Ribosomal Protein L30, Ribosomal Protein L36a, Ribosomal Protein L39L, Ribosomal Protein L4, Ribosomal Protein L9, Ribosomal Protein LP0 and Ribosomal Protein S10

p-Ribosomal Protein S6 Antibody (24.Ser 240) is a mouse monoclonal IgG1 antibody that detects Ribosomal Protein S6 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Ribosomal Protein S6 antibody (24.Ser 240) plays a crucial role in studying the phosphorylation status of Ribosomal Protein S6, which is a key regulator of protein synthesis and cell growth. The phosphorylation of RPS6 is particularly important as it is stimulated by growth factors, tumor-promoting agents, and mitogens, indicating its involvement in cellular signaling pathways that control cell proliferation and metabolism. The conservation of RPS6 across evolutionarily distant eukaryotes, such as yeast and humans, underscores its fundamental role in ribosomal function and protein synthesis. Understanding the dynamics of RPS6 phosphorylation can provide insights into various biological processes, including the response to cellular stress and the regulation of translation, making Ribosomal Protein S6 monoclonal antibody (24.Ser 240) an essential tool for researchers investigating these critical pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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p-Ribosomal Protein S6 Antibody (24.Ser 240) References:

Protein kinase C phosphorylates ribosomal protein S6 kinase betaII and regulates its subcellular localization. | Valovka, T., et al. 2003. Mol Cell Biol. 23: 852-63. PMID: 12529391
Ribosomal protein S6 is a selective mediator of TRAIL-apoptotic signaling. | Jeon, YJ., et al. 2008. Oncogene. 27: 4344-52. PMID: 18362888
Ribosomal protein s6-ps240 is expressed in lesional skin from patients with autoimmune skin blistering diseases. | Abreu-Velez, AM., et al. 2013. N Am J Med Sci. 5: 604-8. PMID: 24350073
Extinction, applied after retrieval of auditory fear memory, selectively increases zinc-finger protein 268 and phosphorylated ribosomal protein S6 expression in prefrontal cortex and lateral amygdala. | Tedesco, V., et al. 2014. Neurobiol Learn Mem. 115: 78-85. PMID: 25196703
A single 60-min bout of peristaltic pulse external pneumatic compression transiently upregulates phosphorylated ribosomal protein s6. | Martin, JS., et al. 2017. Clin Physiol Funct Imaging. 37: 602-609. PMID: 26769680
Phosphorylation of ribosomal protein S6 is dependent on cyclic AMP in Dictyostelium discoideum. | Silva, AM., et al. Second Messengers Phosphoproteins. 12: 271-80. PMID: 2856112
The phosphorylation of eukaryotic ribosomal protein S6 by protein kinase C. | Parker, PJ., et al. 1985. Eur J Biochem. 148: 579-86. PMID: 3158521
Phosphorylation of ribosomal protein S6 during lens cell differentiation: correlation with translational efficiency. | Vu, ND. and Zelenka, P. 1987. Curr Eye Res. 6: 703-8. PMID: 3297499
Protease-activated kinase II as the potential mediator of insulin-stimulated phosphorylation of ribosomal protein S6. | Perisic, O. and Traugh, JA. 1983. J Biol Chem. 258: 9589-92. PMID: 6350281
Ordered phosphorylation of 40S ribosomal protein S6 after serum stimulation of quiescent 3T3 cells. | Martin-Pérez, J. and Thomas, G. 1983. Proc Natl Acad Sci U S A. 80: 926-30. PMID: 6573662

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	RPS6	Entrez Gene ID: 6194	9p22.1	NM_001010	UniProt ID: P62753	180460
Mouse	Rps6	Entrez Gene ID: 20104	4 C4	NM_009096	UniProt ID: P62754	N/A

Ordering Information

Product Name	Catalog #	UNIT	Price	Qty	FAVORITES
p-Ribosomal Protein S6 Antibody (24.Ser 240)	sc-293143	200 µg/ml	$322.00
p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgG Fc BP-HRP Bundle	sc-537721	200 µg Ab; 10 µg BP	$361.00
p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgGκ BP-HRP Bundle	sc-535084	200 µg Ab; 40 µg BP	$361.00
p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgG₁ BP-HRP Bundle	sc-545329	200 µg Ab; 20 µg BP	$361.00

Are there any issues with using sc-293143: p-Ribosomal Protein S6 (24.Ser 240) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-293143 conjugates.

Answered by: Technical Support

Date published: 2017-03-07

Rated 4 out of 5 by Dandave from Excellent antibody!!!We used this antibody to selectively detect the phosphorylation of ribosomal protein S6 by p70 S6K in PC-3 cells by Western blot. When used at 1:1000 (in TBST-3% BSA) this antibody yields a single band at the expected MW. Inhibiting mTOR with rapamycin or YM155, as expected, completely ihibits the signal.

Date published: 2020-04-12

Rated 5 out of 5 by Mariabir from Works perfectyTried a free sample followed up by a purchase of this antibody. Works perfecty in free floating sections for immunohistology and shows beautiful band in WB using mouse brain tissue (band around 37), both in 1:1000.

Date published: 2018-03-15

Rated 4 out of 5 by LR from Worked well with untreatedWorked well with untreated , EGF treated and EGF and lambda protein phosphatase treated HEK293 whole cell lysates by WB with some background. -SCBT QC

Date published: 2014-09-04

p-Ribosomal Protein S6 Antibody (24.Ser 240) is rated 4.3 out of 5 by 3.

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p-Ribosomal Protein S6 Antibody (24.Ser 240): sc-293143

p-Ribosomal Protein S6 Antibody (24.Ser 240) References:

The preferred Ribosomal Protein S6 antibody is Ribosomal Protein S6 Antibody (C-8): sc-74459.

p-Ribosomal Protein S6 Antibody (24.Ser 240)

p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgG Fc BP-HRP Bundle

p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgGκ BP-HRP Bundle

p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgG1 BP-HRP Bundle

Are there any issues with using sc-293143: p-Ribosomal Protein S6 (24.Ser 240) mouse monoclonal primary antibody on mouse samples?

p-Ribosomal Protein S6 Antibody (24.Ser 240): m-IgG₁ BP-HRP Bundle