Anticorpo p-Atm (10H11.E12) raccomandato per il rilevamento di Ser 1981 phosphorylated Atm di origine mouse, rat e human in WB, IP, IF e IHC(P)

Anticorpo p-Atm (10H11.E12): sc-47739

Anticorpo p-Atm (10H11.E12) valutazione 4.5 di 5 di 17.
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Schede tecniche

    • p-Atm Antibody 10H11.E12 è un monoclonale di topo IgG1 κ, citato in 93 pubblicazioni, fornito in 200 µg/ml
    • raised against a synthetic phosphopeptide corresponding to amino acids Ser 1981 surrounding the Ser 1981 phosphorylation site of human ATM
    • p-Atm Antibody (10H11.E12) é raccomandato per il rilevamento di Ser 1981 phosphorylated Atm di origine mouse, rat e human in WB, IP, IF e IHC(P)
    • p-Atm Antibody (10H11.E12) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di p-Atm (10H11.E12): sc-47739.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Atm Antibody (10H11.E12) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Atm Antibody (10H11.E12) (see ordering information below).
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What is a good negative control to test for phospho-specificity of this antibody?

Richiesta da: TinTin
Thank you for your question. We recommend treating your samples with lambda protein phosphatase (sc-200312A) as a control. Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins. As indicated in the Western blot data on our website and datasheet for this antibody (p-Atm Antibody (10H11.E12): sc-47739), treated samples should show a negative result when using this phospho-specific antibody in WB compared to the control phosphorylated sample.
Risposta da: Technical Support
Data di pubblicazione: 2016-12-30
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Rated 5 di 5 di da 功能很好 我很想试试这个抗体表达,很好用,实验必须用到,一直想买的抗体,这个有试用还挺好的,非常感谢,非常好的公司
Data di pubblicazione: 2021-05-23
Rated 5 di 5 di da Very good I tested this antibody in a context of DNA damage induction and it worked very well, giving a strong positive signal in human HeLa cells treated with bleomycin (using a dilution of 1:200 for imunocytochemistry)
Data di pubblicazione: 2021-02-10
Rated 4 di 5 di da good antibody It worked nicely with my human cell lysates, better than I had from other company
Data di pubblicazione: 2018-01-29
Rated 4 di 5 di da good antibody This antibody is good for immunofluorescence. (used at 1:100 dilution)
Data di pubblicazione: 2017-09-29
Rated 4 di 5 di da Good antibody for pAtm I tested a sample antibody and it worked well in immunofluorescence.
Data di pubblicazione: 2017-09-24
Rated 5 di 5 di da Get's the WB done. Worked at a 1:200 dilution for western blot when using a Bio-Rad system and got a cleat band.
Data di pubblicazione: 2017-09-14
Rated 5 di 5 di da This antibody can do a good job. we need to test DNA damage after Etoposide treat, we find this antibody is great, the band is clean and specific at 1:500.
Data di pubblicazione: 2017-09-13
Rated 5 di 5 di da good for ICC This antibody is very good for Immunocytochemistry showing effect of ATM on cell signaling. Used at 1:200 conc.
Data di pubblicazione: 2017-08-20
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