Anticorpo p-Akt1/2/3 (C-11) raccomandato per il rilevamento di Ser 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and correspondingly Ser 472 phosporylated Akt3 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo p-Akt1/2/3 (C-11): sc-514032

Anticorpo p-Akt1/2/3 (C-11) valutazione 4.5 di 5 di 56.
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Schede tecniche

    • p-Akt1/2/3 Antibody C-11 è un monoclonale di topo IgG2b κ, citato in 95 pubblicazioni, fornito in 200 µg/ml
    • specific for an epitope mapping to a short amino acid sequence containing phosphorylated Ser 473 of Akt1 of human origin
    • p-Akt1/2/3 Antibody (C-11) é raccomandato per il rilevamento di Ser 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and correspondingly Ser 472 phosporylated Akt3 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • p-Akt1/2/3 Antibody (C-11) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di p-Akt1/2/3 (C-11): sc-514032.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for p-Akt1/2/3 Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Akt1/2/3 Antibody (C-11) (see ordering information below).
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HRP 의 경우 타겟 항체에 대한 specific 제품을 사용해야 하나요 ?  p-Akt 항체를 가지고 WB를 하려고 하는데, p-Akt1/2/3 Antibody (C-11) HRP 또는 m-IgGκ BP-HRP sc-516102을 사용해도 되는지요 ? 

Richiesta da: seong Joseph
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다.HRP 결합 1 차 항체 있으시면 ,2 차 항체 사용할 필요가 없습니다.sc-514032는 sc-516102을 사용해도 됍니다.다른 문제 있으시면 기술지원팀에 연락하셔서 전화:86 21-60936350 이메일: [email protected] 또는 온라인문의로 문의하시면 됩니다. Thank you for your question.There is no need to use 2nd antibody with HRP conjugated primary antibody.sc-51610 is a suitble 2nd antibody for sc-514032 in WB.If you have any other question ,please contact our Technical Service Department at email [email protected],call us directly at  (86 21) 6093-6350, or contact us by live chat.
Risposta da: Technical Support 8
Data di pubblicazione: 2019-01-25

I am using p-Akt1/2/3 (C-11): sc-514032 monoclonal antibody for Western blot. How do I know if the band I am detecting is specific?

Richiesta da: jenni
We recommend using the blocking peptide sc-514032 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Risposta da: Technical Support 10
Data di pubblicazione: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p-Akt1/2/3 (C-11): sc-514032?

Richiesta da: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 10
Data di pubblicazione: 2017-01-12
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Rated 5 di 5 di da Very good The antibody was used for Western Blot in breast carcinoma cells, it works very well at a concentration of 1: 100
Data di pubblicazione: 2020-10-16
Rated 4 di 5 di da Very good antibody The antibody was used for Western Blot with breast carcinoma cells, it works very well at a concentration of 1: 200.
Data di pubblicazione: 2018-11-22
Rated 5 di 5 di da It works great for western blot. sc-514032 works great for western blot with HUVECs cell lines.
Data di pubblicazione: 2018-11-12
Rated 5 di 5 di da Very good Good on IHC staining of tumor epithelial cells, 1:100 dilution.
Data di pubblicazione: 2018-07-24
Rated 5 di 5 di da great bands it's works great on my western blot! clear bands at 58kDa
Data di pubblicazione: 2018-04-03
Rated 2 di 5 di da Couldn't get it to work for WB Maybe this could work with more optimization. But I ran out of sample so could not optimize. I was using 1:200 dilution in mouse samples.
Data di pubblicazione: 2018-03-22
Rated 5 di 5 di da Funziona molto bene Ho ordinato il capione di prova e subito l'ho provato. Ho diluito l'anticorpo 1:500 in PBS+BSA. Il segnale che ho ottenuto è stato forte e pulito, solo un aspecifico sopra i 90 KDa. Un buon anti mouse, consigliato!
Data di pubblicazione: 2018-01-19
Rated 4 di 5 di da gute Ergebniss in WB. gute Ergebnis in WB. I need to adjust the result a Little to get clear band, but this product do work well for WB.
Data di pubblicazione: 2018-01-15
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