osteopontin/OPN/SPP1 Antibody (AKm2A1): sc-21742

OPN Antibody (AKm2A1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Osteopontin (OPN) in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-OPN antibody (AKm2A1) is available in non-conjugated and conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Osteopontin, also known as bone sialoprotein 1, urinary stone protein, SPP1, Eta-1, nephropontin, and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein that plays a crucial role in various biological processes. Post-translational modifications of OPN, such as phosphorylation and glycosylation, significantly influence molecular weight and functional properties, enabling interactions with specific receptors and proteins to modulate cell adhesion, migration, and immune responses. OPN monoclonal antibody (AKm2A1) helps researchers study this protein, which is predominantly produced by osteoblasts and synthesized in brain and kidneys, demonstrating importance in both skeletal and non-skeletal tissues. The diverse functions of OPN, enhanced by structural variations from post-translational modifications, highlight significance in health and disease, making OPN (AKm2A1) antibody valuable for studying various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

osteopontin/OPN/SPP1 Antibody (AKm2A1) References:

1. Osteopontin, a substrate for transglutaminase and factor XIII activity.  |  Prince, CW., et al. 1991. Biochem Biophys Res Commun. 177: 1205-10. PMID: 1676261
2. Definition of a specific interaction between the early T lymphocyte activation 1 (Eta-1) protein and murine macrophages in vitro and its effect upon macrophages in vivo.  |  Singh, RP., et al. 1990. J Exp Med. 171: 1931-42. PMID: 2351930
3. The nature and significance of osteopontin.  |  Butler, WT. 1989. Connect Tissue Res. 23: 123-36. PMID: 2698313
4. Normal rat kidney cells secrete both phosphorylated and nonphosphorylated forms of osteopontin showing different physiological properties.  |  Nemir, M., et al. 1989. J Biol Chem. 264: 18202-8. PMID: 2808373
5. Structural and functional domains of osteopontin.  |  Butler, WT. 1995. Ann N Y Acad Sci. 760: 6-11. PMID: 7785926
6. Osteopontin: a protein with diverse functions.  |  Denhardt, DT. and Guo, X. 1993. FASEB J. 7: 1475-82. PMID: 8262332
7. Osteopontin at mineralized tissue interfaces in bone, teeth, and osseointegrated implants: ultrastructural distribution and implications for mineralized tissue formation, turnover, and repair.  |  McKee, MD. and Nanci, A. 1996. Microsc Res Tech. 33: 141-64. PMID: 8845514
8. The immunology of Eta-1/osteopontin.  |  Weber, GF. and Cantor, H. 1996. Cytokine Growth Factor Rev. 7: 241-8. PMID: 8971479

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-osteopontin/OPN/SPP1 Antibody (AKm2A1) has 369 citations in a variety of scientific publications.

Primary antibodies like Anti-osteopontin/OPN/SPP1 Antibody (AKm2A1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

osteopontin/OPN/SPP1 Antibody (AKm2A1) Specifications

Alternate Product Name: Osteopontin

Product Clone Name: AKm2A1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: OPN

Epitope Species: mouse

Product Reactivity/Detection: OPN

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 369 citations in various scientific publications

Available Conjugates/Forms for osteopontin/OPN/SPP1 Antibody (AKm2A1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred osteopontin/OPN/SPP1 antibody is osteopontin/OPN/SPP1 Antibody (LFMb-14): sc-73631.