Odf2 Antibody (G-8): sc-365874
- Odf2 Antibody (G-8) is a mouse monoclonal IgG1 κ Odf2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 3-27 at the N-terminus of Odf2 of human origin
- Odf2 Antibody (G-8) is recommended for detection of Odf2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- Anti-Odf2 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Odf2 Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Odf2 Antibody (G-8) (see ordering information below).
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Odf2 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Odf2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Odf2 antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Odf2 plays a crucial role in the structural integrity of the outer dense fibers (ODF) in the mammalian sperm tail, which are essential for maintaining the elasticity and proper function of the flagellum during sperm motility. These fibers are located outside the axoneme and are vital for the passive elastic properties and recoil of the sperm tail, which are necessary for effective swimming. ODFs are composed of a complex array of proteins, including Odf1, Odf2, and Odf3, which are predominantly expressed during spermiogenesis. Notably, Odf2 interacts with Odf1 through leucine zipper motifs, facilitating the assembly of the outer dense fibers, while Odf1 can also self-interact. Proper functioning of these proteins is critical, as abnormalities in their expression or interaction may contribute to male infertility due to flagellar dysfunction. The human Odf2 gene is located on chromosome 9q34, and both Odf1 and Odf2 are exclusively expressed in the testis, highlighting their specialized role in reproductive biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Odf2 Antibody (G-8) References:
- Human outer dense fiber gene, ODF2, localizes to chromosome 9q34. | Shao, X., et al. 1998. Cytogenet Cell Genet. 83: 221-3. PMID: 10072582
- Outer dense fibre proteins from human sperm tail: molecular cloning and expression analyses of two cDNA transcripts encoding proteins of approximately 70 kDa. | Petersen, C., et al. 1999. Mol Hum Reprod. 5: 627-35. PMID: 10381817
- Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1. | Shao, X., et al. 2001. Mol Reprod Dev. 59: 410-6. PMID: 11468777
- Keratins: unraveling the coordinated construction of scaffolds in spermatogenic cells. | Kierszenbaum, AL. 2002. Mol Reprod Dev. 61: 1-2. PMID: 11774369
- Sequence, expression, and chromosomal assignment of a human sperm outer dense fiber gene. | Gastmann, O., et al. 1993. Mol Reprod Dev. 36: 407-18. PMID: 8305202
- Self-interaction of the major 27-kilodalton outer dense fiber protein is in part mediated by a leucine zipper domain in the rat. | Shao, X. and van der Hoorn, FA. 1996. Biol Reprod. 55: 1343-50. PMID: 8949892
- Developmental expression of the 84-kDa ODF sperm protein: localization to both the cortex and medulla of outer dense fibers and to the connecting piece. | Schalles, U., et al. 1998. Dev Biol. 199: 250-60. PMID: 9698445
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Odf2 Antibody (G-8) | sc-365874 | 200 µg/ml | $322.00 | |||
Odf2 Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-525904 | 200 µg Ab; 10 µg BP | $361.00 | |||
Odf2 Antibody (G-8) AC | sc-365874 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Odf2 Antibody (G-8) HRP | sc-365874 HRP | 200 µg/ml | $322.00 | |||
Odf2 Antibody (G-8) FITC | sc-365874 FITC | 200 µg/ml | $336.00 | |||
Odf2 Antibody (G-8) PE | sc-365874 PE | 200 µg/ml | $349.00 | |||
Odf2 Antibody (G-8) Alexa Fluor® 488 | sc-365874 AF488 | 200 µg/ml | $364.00 | |||
Odf2 Antibody (G-8) Alexa Fluor® 546 | sc-365874 AF546 | 200 µg/ml | $364.00 | |||
Odf2 Antibody (G-8) Alexa Fluor® 594 | sc-365874 AF594 | 200 µg/ml | $364.00 | |||
Odf2 Antibody (G-8) Alexa Fluor® 647 | sc-365874 AF647 | 200 µg/ml | $364.00 | |||
Odf2 Antibody (G-8) Alexa Fluor® 680 | sc-365874 AF680 | 200 µg/ml | $364.00 | |||
Odf2 Antibody (G-8) Alexa Fluor® 790 | sc-365874 AF790 | 200 µg/ml | $364.00 | |||
Odf2 (G-8) Neutralizing Peptide | sc-365874 P | 100 µg/0.5 ml | $69.00 |