Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mucin 1/MUC1 Double Nickase Plasmid (h): sc-416876-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 1/MUC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mucin 1/MUC1 Double Nickase Plasmid (h) and Mucin 1/MUC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MUC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mucin 1/MUC1 Antibody (VU4H5): sc-7313
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 1/MUC1 Double Nickase Plasmid (h) | sc-416876-NIC | 20 µg | $410.00 | |||
Mucin 1/MUC1 Double Nickase Plasmid (h2) | sc-416876-NIC-2 | 20 µg | $410.00 |
MUC1 encodes mucin 1, a heavily O-glycosylated type I transmembrane mucin expressed on the apical surface of many epithelial tissues where it contributes to barrier function, hydration, and protection from environmental stress. Through its cytoplasmic tail, MUC1 engages signaling networks that regulate cell polarity, adhesion, and transcriptional responses, intersecting with pathways such as receptor tyrosine kinase signaling and downstream MAPK/PI3K-associated processes. Altered MUC1 expression, localization, or glycoforms are frequently linked to disrupted epithelial organization and immune interactions in inflammatory and neoplastic contexts. As a result, MUC1 is widely studied as a modulator of epithelial homeostasis, tumor cell biology, and mucin-dependent microenvironmental signaling.
Mucin 1/MUC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MUC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MUC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MUC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MUC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.