Anticorpo MAP LC3 beta (G-2) raccomandato per il rilevamento di MAP LC3β and MAP LC3β2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo MAP LC3β (G-2): sc-271625

Anticorpo MAP LC3β (G-2) valutazione 4.5 di 5 di 35.
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Schede tecniche

    • MAP LC3 beta Antibody G-2 è un monoclonale di topo IgG2b κ, citato in 55 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 1-50 mappati al N-terminus di MAP LC3β di origine human
    • MAP LC3 beta Antibody (G-2) é raccomandato per il rilevamento di MAP LC3β and MAP LC3β2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse e canine, bovine and porcine
    • MAP LC3 beta Antibody (G-2) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di MAP LC3β (G-2): sc-271625.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAP LC3β Antibody (G-2) for WB and IHC(P) applications. These reagents are now offered in bundles with MAP LC3β Antibody (G-2) (see ordering information below).
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would you recommend blocking in milk or BSA for western blots? or something else? Thanks

Richiesta da: SaCam
Thank you for your question. We recommend blocking in 5% nonfat milk in 1X TBS with 0.01% Tween-20. Please see our Western blotting protocol online for more detailed recommendations.
Risposta da: Technical Support
Data di pubblicazione: 2019-05-01

What is the recommended secondary reagent to be used with MAP LC3β (G-2): sc-271625 antibody for immunofluorescence?

Richiesta da: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins (m-IgGκ) as a secondary detection reagent that is conjugated to PE, FITC or a CruzFluor. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 8
Data di pubblicazione: 2017-01-09
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Rated 5 di 5 di da 效果很好 I bought this antibody a month ago, it is good, i will buy next time
Data di pubblicazione: 2018-11-25
Rated 5 di 5 di da Inflammasome by the hypercaloric diets We used this antibody to evaluate the autophagy role in the heart damage induced by hypercaloric diets
Data di pubblicazione: 2018-10-28
Rated 5 di 5 di da Excellent I used it with 1:1000 dilution. It works really good with cell lysate. Some non-specific band. However, very clear band on the target MW.
Data di pubblicazione: 2018-10-10
Rated 5 di 5 di da works very good in WB I used this antibody in WB in human fibroblasts. I got great signals for the LC3I and LC3II bands. Also in combination with Rapamycin, an autophagy inducer, the expected results were examined.
Data di pubblicazione: 2018-06-06
Rated 5 di 5 di da Works well I use this antibody for WB and IF on HEK293 cells in the Hinck Lab, and I see great results every time.
Data di pubblicazione: 2018-05-09
Rated 5 di 5 di da Excellent for ICC I received this antibody as a sample and used it for ICC on rat primary neurons. We used the antibody at 1:50 and the secondary antibody was donkey anti-mouse Alexa 488 at 1:1000. We took images at 30X. It worked the best of all the antibodies we tried
Data di pubblicazione: 2018-04-11
Rated 2 di 5 di da Weak signal on LC3I Weak signal on LC3I only. Also only works in mouse muscle cells and not mouse muscle tissue.
Data di pubblicazione: 2018-02-22
Rated 2 di 5 di da Not work in IF staining I tried the antibody (free sample) in the endothelial cells with 4%PFA fixation and 1:50 dilution, but it did not give me a good result.
Data di pubblicazione: 2018-01-31
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