Anticorpo MAD1 (D-5) raccomandato per il rilevamento di MAD1 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo MAD1 (D-5): sc-166313

Anticorpo MAD1 (D-5) valutazione 5.0 di 5 di 1.
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Schede tecniche

    • MAD1 Antibody D-5 è un monoclonale di topo IgG2a fornito in 200 µg/ml
    • raised against amino acids 491-718 mapping at the C-terminus of MAD1 of human origin
    • raccomandato per il rilevamento di MAD1 di origine mouse, rat e human in WB, IP, IF e ELISA
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAD1 Antibody (D-5) for WB applications. These reagents are now offered in bundles with MAD1 Antibody (D-5) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using MAD1 (D-5): sc-166313?

Richiesta da: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-03-01
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Rated 5 di 5 di da Great Western Blot data of MAD1 expression in non Great Western Blot data of MAD1 expression in non-transfected 293T, mouse MAD1 transfected 293T and HeLa whole cell lysates. -SCBT QC
Data di pubblicazione: 2014-02-04
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