LPS A mitogen for bone marrow derived B lymphocytes.

LPS

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LPS is rated 5.0 out of 5 by 6.
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Nomi alternativi: LPS is also known as Lipopolysaccharide.
Applicazione: LPS is a highly immunogenic major component of the cell wall of gram negative bacteria that strongly activates immune cells bearing the CD14/TLR4/MD2 rec
Purezza: purified by phenol extraction
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
* Vedere Certificato di Analisi per informazioni sul lotto specifico (incluso il contenuto d'acqua).
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LPS (Lipopolysaccharide) is an endotoxin which exists as a critical glycolipid component of the outer cell wall of gram-negative bacteria. LPS is a known, potent initiator of the proinflammatory response and induces proinflammatory cytokines in several cell types. Many of the cell signals induced by gram-negative bacteria are attributed to LPS and has been observed to also activate TNF receptor signaling pathways. The major receptor that transduces the LPS signal has been recognized as a member of the Toll-like receptor family. At least 10 members of the family have been identified to date and TLR4 appears to be the main LPS receptor although coreceptors are most likely involved. Studies note that in HMEC (human microvascular endothelial cells) cell lines, LPS activates apoptosis pathways via both NF-κB and JNK through TRAF6. Also reported that NF-κB inhibition does not potentiate LPS-induced death in HMEC cells yet will sensitive these cell to TNF-induced death. Additional studies using HMEC cell lines report that following LPS stimulation, TRAF6 also transmits an important endothelial cell survival signal in a situation of complete NF-κB blockade which may indicate that TRAF6 signals an NF-κB–independent antiapoptotic pathway in endothelial cells exposed to LPS. LPS is also known as Lipopolysaccharides, Lipopolysaccharides from E. coli O111:B4, and Bacterial lipopolysaccharides.


Referenze

1. Zhang, F.X., et al. 1999. J. Biol. Chem. 274: 7611-7614. PMID: 10075645

2. Hull, C., et al. 2002. J. Immunol. 169: 2611-2618. PMID: 12193732

3. Raschi, E., et al. 2003. Blood. 101: 3495-3500. PMID: 12531807

4. Pollet, I., et al. 2003. Blood. 102: 1740-1742. PMID: 12714497

5. Wong, F., et al. 2004. Blood. 103: 4520-4526. PMID: 14996708

6. Wang, J., et al. 2008. J. Immunol. 180: 8011-8019. PMID: 18523264

Utilizzo :
For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solution or cell culture medium.
Storage/Stability:
Solutions at 1 mg/ml in buffer or culture medium are stable for ~1 month at 2–8° C. Frozen aliquots can be stored up to 2 years. Repeated freeze/thaw cycles are not recommended. Solutions should be stored in silanized containers, since LPS can bind to plastics and certain types of glass (especially at concentrations of <0.1 mg/ml). If the LPS concentration is >1 mg/ml, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve the adsorbed product.
Aspetto :
Lyophilized powder
Stato fisico :
Solid
Derivato da :
E. coli O111:B4
Solubilità :
Soluble in water (5 mg/ml), and cell culture medium (1 mg/ml).
CONSERVAZIONE :
Store at 4° C
Solo per uso in Ricerca. Non previsto per Uso Diagnostico o Terapeutico.
WGK Germania :
3
PubChem CID :
Numero MDL :
MFCD00164401
SMILES :
CCCCCCCCCCCCCC(=O)O[[email protected]](CCCCCCCCCCC)CC(=O)O[[email protected]@H]1[[email protected]]([[email protected]@H](O[[email protected]@H]([[email protected]]1OP(=O)(O)O)CO[[email protected]@]2(C[[email protected]]([[email protected]@H]([[email protected]](O2)[[email protected]](CO)O)OC3[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O3)[[email protected]](CO)O)OP(=O)(O)OP(=O)(O)OCCN)OC4[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O4)[[email protected]](COC5[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O5)[[email protected]](CO)O)OP(=O)(O)O)O)O)O)O)OC6[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O6)CO[[email protected]@H]7[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O7)CO)O)O)O)O)O[[email protected]@H]8[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O8)CO)O)O)OC9[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O9)CO)O)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O)O)NC(=O)C)O)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO)O)O[[email protected]@H]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO[[email protected]@H]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO)O)O)O)O)O)O)NC(=O)C)NC(=O)C)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O)O)NC(=O)C)O)O)O)O[[email protected]@]1(C[[email protected]]([[email protected]@H]([[email protected]](O1)[[email protected]](CO)O)O)O[[email protected]@]1(C[[email protected]]([[email protected]@H]([[email protected]](O1)[[email protected]](CO)O)O)OP(=O)(O)OCCN)C(=O)O)C(=O)O)C(=O)O)OC[[email protected]@H]1[[email protected]]([[email protected]@H]([[email protected]]([[email protected]](O1)OP(=O)(O)O)NC(=O)C[[email protected]@H](CCCCCCCCCCC)O)OC(=O)C[[email protected]@H](CCCCCCCCCCC)O)O)NC(=O)C[[email protected]@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC

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LPS  Citazioni prodotti

Vedi quanti altri hanno usato LPS. Clicca sulla voce per laccesso a PubMed .

Ciatazioni 1 a10 di 73 totali

PMID: # 32135404  Xu, X.|Hu, P.|Ma, Y.|Tong, L.|Wang, D.|Wu, Y.|Chen, Z.|Huang, C.| et al. 2020. J Nutr Biochem. 78: 108323.

PMID: # 30938885  Yang, J.|Li, R.|Zhao, D.|Zheng, S.| et al. 2019. J. Cell. Biochem. 120: 12887-12903.

PMID: # 30914954  Rossaneis, AC.|Longhi-Balbinot, DT.|Bertozzi, MM.|Fattori, V.|Segato-Vendrameto, CZ.|Badaro-Garcia, S.|Zaninelli, TH.|Staurengo-Ferrari, L.|Borghi, SM.|Carvalho, TT.|Bussmann, AJC.|Gouveia, FS.|Lopes, LGF.|Casagrande, R.|Verri, WA.| et al. 2019. Front Pharmacol. 10: 229.

PMID: # 29581852  Wang, Q.|Yang, X.|Xu, Y.|Shen, Z.|Cheng, H.|Cheng, F.|Liu, X.|Wang, R.| et al. 2018. Oncotarget. 9: 14397-14412.

PMID: # 29058362  Huang, C. et al. 2018. Glia. 66: 256-278.

PMID: # 30247686  Miklavcic, JJ.|Badger, TM.|Bowlin, AK.|Matazel, KS.|Cleves, MA.|LeRoith, T.|Saraf, MK.|Chintapalli, SV.|Piccolo, BD.|Shankar, K.|Yeruva, L.| et al. 2018. J. Nutr. 148: 1860-1870.

PMID: # 29844724  Al-Saeedan, AS.|Gautam, V.|Ansari, MN.|Singh, M.|Yadav, RK.|Rawat, JK.|Devi, U.|Gautam, S.|Roy, S.|Kaithwas, G.| et al. 2018. Saudi Pharm J. 26: 520-527.

PMID: # 28892097  Muku, GE.|Lahoti, TS.|Murray, IA.|Podolsky, MA.|Smith, KJ.|Hubbard, TD.|Kuzu, G.|Gowda, K.|Amin, SG.|Perdew, GH.| et al. 2017. Lab Invest. 97: 1471-1487.

PMID: # 28515460  Giakoumelou, S.|Wheelhouse, N.|Brown, J.|Wade, J.|Simitsidellis, I.|Gibson, D.|Saunders, PTK.|Horner, P.|Entrican, G.|Howie, SEM.|Horne, AW.| et al. 2017. Sci Rep. 7: 2001.

PMID: # 27753084  Ren, JP. et al. 2017. Immunology. 150: 213-220.

Ciatazioni 1 a10 di 73 totali
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