Anticorpo LEF-1 (2D12) raccomandato per il rilevamento di LEF-1 di origine mouse, rat e human in WB e IP

Anticorpo LEF-1 (2D12): sc-81470

Anticorpo LEF-1 (2D12) valutazione 4.0 di 5 di 1.
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Schede tecniche

    • LEF-1 Antibody 2D12 è un monoclonale di topo IgG1, citato in 2 pubblicazioni, fornito in 50 µg/0.5 ml
    • generato contro gli amminoacidi 1-85 di recombinant LEF-1 di origine human
    • raccomandato per il rilevamento di LEF-1 di origine mouse, rat e human in WB e IP
    • Vedere LEF-1 (B-10): sc-374412 for LEF-1 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for LEF-1 Antibody (2D12). This work is in progress.
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Could this antibody work for FA fixed chromatin immunoprecipitation?

Richiesta da: Anonimo
Thank you for your question. LEF-1 Antibody (2D12): sc-81470 is only recommended for detection of LEF-1 of mouse, rat and human origin by WB and IP.
Risposta da: Tech Support Europe
Data di pubblicazione: 2020-08-25

I am using LEF-1 (2D12): sc-81470 antibody for immunoprecipitation, how much antibody should I use?

Richiesta da: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Risposta da: Technical Support
Data di pubblicazione: 2017-03-24
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Rated 4 di 5 di da Positive results in western blot using human LEF Positive results in western blot using human LEF-1 transfected lysates. -SCBT QC
Data di pubblicazione: 2014-02-26
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