LEF-1 Antibody (2D12): sc-81470
- LEF-1 Antibody (2D12) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/0.5 ml
- raised against amino acids 1-85 of LEF-1 of human origin
- recommended for detection of LEF-1 of mouse, rat and human origin by WB and IP
- See LEF-1 (B-10): sc-374412 for LEF-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for LEF-1 Antibody (2D12). This work is in progress.
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LEF-1 Antibody (2D12) is a mouse monoclonal IgG1 antibody that detects LEF-1 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). LEF-1, a member of the TCF/LEF family of transcription factors, plays a crucial role in the Wnt signaling pathway, which is essential for various developmental processes and cellular functions. LEF-1 is primarily located in the nucleus, where LEF-1 interacts with β-catenin to regulate the transcription of Wnt target genes. Proper localization of LEF-1 ensures transcriptional activation of these genes occurs in response to Wnt signaling, influencing cell fate decisions and maintaining stem cell properties. Dysregulation of LEF-1 and associated pathways can lead to developmental disorders and contribute to the progression of certain cancers, highlighting the significance of studying LEF-1 in both developmental biology and cancer research. Anti-LEF-1 antibody (2D12) serves as an invaluable tool for researchers investigating the intricate mechanisms of Wnt signaling and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LEF-1 Antibody (2D12) References:
- Overexpression of beta-catenin induces apoptosis independent of its transactivation function with LEF-1 or the involvement of major G1 cell cycle regulators. | Kim, K., et al. 2000. Mol Biol Cell. 11: 3509-23. PMID: 11029052
- Neutrophil elastase is severely down-regulated in severe congenital neutropenia independent of ELA2 or HAX1 mutations but dependent on LEF-1. | Skokowa, J., et al. 2009. Blood. 114: 3044-51. PMID: 19620402
- TCF-1 and LEF-1 help launch the T(FH) program. | Kubo, M. 2015. Nat Immunol. 16: 900-1. PMID: 26287589
- LEF-1 Regulates Tyrosinase Gene Transcription In Vitro. | Wang, X., et al. 2015. PLoS One. 10: e0143142. PMID: 26580798
- CDX2 and LEF-1 expression in pilomatrical tumors and their utility in the diagnosis of pilomatrical carcinoma. | Tumminello, K. and Hosler, GA. 2018. J Cutan Pathol. 45: 318-324. PMID: 29369390
- LEF1 haploinsufficiency causes ectodermal dysplasia. | Lévy, J., et al. 2020. Clin Genet. 97: 595-600. PMID: 32022899
- Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas. | Heino, S., et al. 2021. Sci Adv. 7: eabj0512. PMID: 34788095
- Usefulness of LEF-1 Immunostaining for the Diagnosis of Matricoma. | Reymundo-Jiménez, A., et al. 2022. Actas Dermosifiliogr. 113: 907-910. PMID: 36202529
- β-Sitosterol blocks the LEF-1-mediated Wnt/β-catenin pathway to inhibit proliferation of human colon cancer cells. | Gu, S., et al. 2023. Cell Signal. 104: 110585. PMID: 36603684
- TCF7 and LEF-1 downregulation in sepsis promotes immune suppression by inhibiting CD4+ T cell proliferation. | Chen, D., et al. 2023. Microb Pathog. 184: 106362. PMID: 37741305
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LEF-1 Antibody (2D12) | sc-81470 | 50 µg/0.5 ml | $322.00 |